in them [85]. The study also found that the administration of 5- AzaC administration improved 5-FU responsiveness in CRC SW480 cell lines [85]. These findings revealed that DNA methylation plays an important role in the development of therapeutic resistance, and targeting it in carcinoma patient...
drugs were chosen to have a high drug distance as well as a high sensitivity in GBM cell lines (Supplementary Figs.1dand3a(showing sensitivity and distance, respectively)). We tested a total of 30 different combinations (Supplementary Data1) onto 9 ...
RESULTS. Predominant drug sensitivities include the highly potent but expected pan-cytotoxic effects of compounds that target microtubules, the proteasome, topoisomerases, DNA/RNA synthesis and histone deacetylases. Beyond these, approximately 50 compounds have been selected for further studies including ...
Paclitaxel (PTX) is a cytotoxic drug extracted from Taxus and enables interaction with β-microtubule proteins, thereby causing the aggregation of microtubules causing cell cycle cessation in the G2/M phase and inducing apoptosis. PTX has shown superior therapeutic effects on cancers including breast,...
and synergized with TMZ to inhibit the growth of TMZ-resistant xenografts. This demonstrated the therapeutic potential of targeting the SOX4/EZH2/METTL3 axis in the treatment of TMZ-resistant glioblastoma (GBM) [43]. Bhattarai et al. showed that METTL3 expression was upregulated in A375R cells...
(Supplementary Fig.14a). Secondly, this signature SBS30L/7bL/11L associates with sensitivity to the vinca alkaloids vinblastine, vincristine, and vinorelbine that interfere with assembly of microtubules and forming of the mitotic spindle (Supplementary Fig.14b). These associations were observed across...
Targeting programmed cell death is a major approach of antitumor drug development. Deregulation of programmed cell death (PCD) contributes to resistance to a variety of cancer therapeutics. Yes-associated protein (YAP) and its paralog TAZ, the main downstream effectors of the Hippo pathway, are ...
Tubulin interactive agents interfere with cell division by binding to specific sites on Tubulin, a protein that polymerizes to form cellular microtubules. Microtubules are critical cell structure units. When the interactive agents bind on the protein, the cell cannot properly form microtubules. Tubulin...
targeting them has proven to be a promising therapeutic approach to overcome resistance. Various reports demonstrated that the expression of Notch1 plays a central role in increasing trastuzumab resistance in BT474, SK-BR3, and MCF-7 cells, and its inhibition (genetic or pharmacological) sensitizes...
3. Drugs That Target the Cytoskeleton in 2D and 3D Cell Culture The cytoskeleton, a dynamic network of polymerized proteins, is crucial for cell division, maintaining shape, and facilitating motility by organizing cellular contents and enabling interaction with the environment. Comprising microtubules, ...