为了验证S616磷酸化通过促进Drp1二聚化促进Drp1与MFF相互作用的假设,对PT130细胞与GFP和Flag标记的Drp1 WT或S616A突变体共转染。事实上,PA增加了两个WT Drp1分子的相互作用,但并未增强Drp1-S616A突变蛋白的相互作用(图2f)。综上所述,...
本研究表明通过调节RIP1/RIP3通路加速p62自噬小体转换和破损线粒体清除可能是改善CIRI恶性循环的有效措施,而维持线粒体质量平衡和减少线粒体ROS堆积(包括减少ROS生成和增加ROS清除等)可能是从根本上改善CIRI预后的关键。 本文中Drp1 干扰腺...
本研究表明通过调节RIP1/RIP3通路加速p62自噬小体转换和破损线粒体清除可能是改善CIRI恶性循环的有效措施,而维持线粒体质量平衡和减少线粒体ROS堆积(包括减少ROS生成和增加ROS清除等)可能是从根本上改善CIRI预后的关键。 本文中Drp1 干扰腺病毒、Drp1-S616D 和 Drp1-S616A 过表达腺病毒及外泌体浓度粒径(NTA)检测...
PINK1-mediated mitochondrial fission is Drp1S616 phosphorylation dependent. Overexpression of either wild-type Drp1 or of the phosphomimetic mutant Drp1S616D , but not a dephosphorylation-mimic mutant Drp1S616A , rescues PINK1 deficiency-associated phenotypes in Drosophila. Moreover, Drp1 restores PINK1-...
Additionally, we found that the cell viability in LMP1-overexpressing CNE1 and HONE1 cells was increased compared with that in control cells, and the effects were suppressed by Drp1 S616A (Fig. 2h). In addition, a significant reduction in cell viability was observed by silencing LMP1 in CNE...
the phosphorylation-deficient mutant Drp1S616A shows less fragmented mitochondria in themutant cells, confirming this serine residue as the site of activation. After testing together the phosphorylation-deficient mutant of Drp1 and the MEK1/2 inhibitor, we confirm that Drp1 fission and MAPK1 phosphoryl...
本文中Drp1 干扰腺病毒、Drp1-S616D 和 Drp1-S616A 过表达腺病毒及外泌体浓度粒径(NTA)检测均由上海吉凯基因提供。 段晨阳,临床医学博士,博士后,重庆医学会麻醉学分会青年委员。长期从事围术期危重症多器官功能损害与保护相关临床工作和科学研究。主持国家自然科学基金青年基金1项、中国博士后面上项目1项,入选 “...
本文中Drp1 干扰腺病毒、Drp1-S616D 和 Drp1-S616A 过表达腺病毒及外泌体浓度粒径(NTA)检测均由上海吉凯基因提供。 段晨阳,临床医学博士,博士后,重庆医学会麻醉学分会青年委员。长期从事围术期危重症多器官功能损害与保护相关临床工作和科学研究。主持国家自然科学基金青年基金1项、中国博士后面上项目1项,入选 “...
In Drp1 deletion fibroblasts, re-expression of wild-type Drp1 rather than Drp1S616A mutant restores the reduction of TGF-β-induced-Drp1 phosphorylation, H3K27ac, and cell activation. Moreover, TGF-β1 treatment increased the enrichment of H3K27ac at the promoters of α-SMA and PCNA, which...
N2a cells were transfected with different plasmids (Drp1-Myc, the dephosphorylation-mimic mutant Drp1S616A-Myc and the phosphorylation-mimic mutant Drp1S616D-Myc). The expression of CDK5 and its activator p35, Drp1 and phosphorylated Drp1 on S616 was determined by western blot. The morphology of...