Drp1的丝氨酸616(S616)磷酸化促进其易位,而Drp1的丝氨酸637(S637)磷酸化则抑制其易位。研究者发现在CSE KO-ISO心脏中,S616处Drp1磷酸化水平较高,S637处Drp1磷酸化水平较低。相反,这些变化在CSE OE-ISO心脏中减弱(图2d, e)。此...
为进一步研究脂质过载所致线粒体形态变化,作者检测了线粒体融合、分裂相关蛋白的表达,发现分裂调节蛋白Drp1在整体细胞及线粒体中增加明显。已知Drp1从胞质到线粒体的转位受翻译后修饰调节,作者分别检测了整体、胞质、线粒体中的Drp1磷酸化水平...
Drp1的丝氨酸616(S616)磷酸化促进其易位,而Drp1的丝氨酸637(S637)磷酸化则抑制其易位。研究者发现在CSE KO-ISO心脏中,S616处Drp1磷酸化水平较高,S637处Drp1磷酸化水平较低。相反,这些变化在CSE OE-ISO心脏中减弱(图2d, e)。此外Drp1多聚体也受到了H2S的调节(图2f, g)。这些结果说明H2S在心力衰竭时调节Drp1...
Drp1从细胞质到线粒体的转移受多种信号和翻译后修饰的调节,这其中Drp1的丝氨酸616(S616)磷酸化促进其移位,而Drp1的丝氨酸637(S637)磷酸化则抑制其移位。实验数据显示,在CSE KO-ISO心脏中,S616处Drp1磷酸化水平较高,S637处Drp1磷酸化...
相比之下,对Drp1活性具有负性调节作用的S637位点在PA处理后并未发生磷酸化。在经PA处理的HCT116细胞和经OA处理的PT130细胞中,也观察到Drp1在S616位点的磷酸化同样也有增加(补充图S1e,f)。然而,在蛋白质和mRNA水平上,FAs对总Drp1和其...
To observe the roles of Drp1 in IDD, we measured the expression of total Drp1, p-Drp1(S616) and p-Drp1(S637) in NP tissues of control group (NC) and degenerative group (IDD). As shown in Fig. 1A, B, the level of Drp1 phosphorylated at Ser616 significantly increased in degenerative...
In this study, we found that Drp1 is phosphorylated at S616 and translocates to mitochondria upon ferroptosis induction hand in hand with an increase in GTPase activity. In necroptosis, PGAM5 has been described to recruit Drp1 and activate its GTPase activity by dephosphorylating P-S637 [22, ...
(S637),phospho-Drp1(S616)和Drp1的表达.结果 CuET对HepG2和SMMC-7721细胞增殖和集落形成的抑制作用呈剂量依赖性(P<0.01);对HepG2和SMMC-7721细胞凋亡诱导作用也有明显的剂量和时间依赖性(P<0.01);Western blot检测结果显示,CuET可导致Caspase 3和Caspase 9的裂解激活,PARP的降解激活以及cyto C从线粒体向细胞...
The following antibodies were used: antibodies against Drp1 (D6C7) (#8570S; WB 1:1000, IF 1:100, IP/PLA 1:100), P-Drp1(S616) (#3455S; WB 1:1000), P-Drp1 (S637) (#4867S; WB 1:1000), Desmin (D93F5) (5332S; WB 1:1000, IF 1:100), eIF2alpha (#9722S; WB 1:1000...
DRP1 phosphorylation at S637 by protein kinase A inhibits fission by enhancing the dissociation of DRP1 from mitochondria. DRP1 phosphorylation at S616 activates mitochondrial fission by cyclin B–CDK1–RALA binding protein 1 (RALBP1) complex.35 Thus, we investi- gated whether LIMK2-mediated ...