We performed RNAi knockdown of the Drosophila orthologs in border cells to evaluate if migration and/or cohesion of the cluster was impaired. From this screen, we identified eight adhesion-related genes that disrupted border cell collective migration upon RNAi knockdown. Bioinformatics analyses further ...
Therefore, we first performed the genome-wide transgenic RNAi screen by the ubiquitous knockdown of genes with Act-Gal4 and then characterized the functions of selected genes in individual cells with cell-type-specific Gal4 (such as germ cells specific driver, Nanos (Nos)-Gal4 or CySC and ...
A genome-wide RNAi screen was carried out using dsRNAs fromDrosophilaHeidelberg 2 (HD2) library, covering about 98% of the annotatedDrosophilagenome [27,28]. Importantly, dsRNAs against known trxG members (trx, ash1) and specific reporter gene (F.Luc) were used as positive controls, whereas ...
Table S4. Screen Analysis, Related to Figure 2 Table S5. Fly Hits that Correspond to Existing Knockout Mice with Cardiovascular Phenotypes, Related to Figure 2 Movie S1. Visualization of Control and not3 RNAi Heart Function, Related to Figure 3Show all extrasVolume...
RNA干扰也称RNAi,是一种应用广泛的技术,它将双链RNA引入到生物体内从而导致目的基因沉默 。 获得诺贝尔奖的RNA干扰技术能让研究人员沉默线虫的任何基因表达从而研究其功能。为将RNAi引入线虫,我们可以先制备表达了目的基因双链RNA的大肠杆菌平板作为线虫的食物。然后将第四幼虫期的线虫转移到RNAi平板上培养并让其产卵。
A global in vivo Drosophila RNAi screen identifies a key role of ceramide phosphoethanolamine for glial ensheathment of axons. PLoS Genet. 9, e1003980 (2013). PubMed PubMed Central Google Scholar Gerdøe-Kristensen, S., Lund, V. K., Wandall, H. H. & Kjaerulff, O. Mactosyl...
extensive study, the issue of false negatives has received less attention./p pResults/p pWe performed a meta-analysis of several genome-wide, cell-based itDrosophila /itRNAi screens, together with a more focused RNAi screen, and conclude that the rate of false negative results is at least 8...
Zuber J, Shi J, Wang E, Rappaport AR, Herrmann H, Sison EA, Magoon D, Qi J, Blatt K, Wunderlich M, Taylor MJ, Johns C, Chicas A, Mulloy JC, Kogan SC, Brown P, Valent P, Bradner JE, Lowe SW, Vakoc CR: RNAi screen identifies Brd4 as a therapeutic target in acute myeloid le...
AGO2 recruitment into chromatin depends on active transcription at a proportion of AGO2-bound promoters. (A) Screenshot showing an example AGO2-dependent geneTraf4-A, where AGO2 ChIP signal is lost upon addition of the transcription inhibitor Triptolide. Black bar corresponds to ChIP-seq peak....
Compared with the controls g and m, ectopic expression of Egr driven by ptc-GAL4 generates a loss-of-ACV phenotype in adults h and cell death in larval wing discs n, which are strongly blocked by RNAi-mediated depletion of sna j, k, p and q, but not that of GFP i and o. The ...