This EcoRI-KpnI fragment was then subcloned into the Drosophila transformation vector pStinger (Drosophila Genomics Resource Center), which contains a multiple cloning site upstream of the nuclear EGFP reading
Paul Schedl, Drosophila Genomics Resource Center. The Duke Light Microscopy Core Provided imaging support. We thank Anne West, Chris Nicchitta, Scott Allen, and Fox lab members for their comments on the manuscript. This project was supported by ACS grant RSG-128945 to D.F., NIH grants R35 ...
The coding sequence of the lacZ gene was cloned into the pUASz1.0 vector (Drosophila Genomics Resource Center, DGRC #1431) (DeLuca and Spradling, 2018). The plasmids were microinjected into the attP40 fly strain to generate site-specific transgenic flies on chromosome 2L. Immunofluorescent staini...
The 3X HA tag was amplified using pTWH (Drosophila Genomics Resource Center; T. Murphy) using Gdrd Rescue F2/R2 primers. PCR fragments were then assembled into a XbaI/AscI-linearized w +attB plasmid (Sekelsky, Addgene plasmid 30326). Tagged rescue construct was then phiC31 integrated into ...
Drosophila S2R+ cells (Drosophila Genomics Resource Center (DGRC)) were cultured at 25 °C in Schneider’s Drosophila Medium (Thermofisher) supplemented with 10% FBS (Biowest) and penicillin/streptomycin (Invitrogen). Antibodies The following antibodies were used for ChIP: dCTCF polyclonal antibodi...
melanogaster proteasome subunit β5 cDNA (Drosophila Genomics Resource Center, NIH 2P40OD010949) into a previously described insertion line (attp33)42 using standard methods. The daughterless-GAL4 driver and w1118 genetic background lines were obtained from the Bloomington Drosophila Stock Center (...
Drosophila Genomics and Genetic Resources DGRC: Drosophila Genomics Resource Center DIOPT: Drosophila RNAi Screening Center Integrative Ortholog Prediction Tool Dl : Delta DLL: DeLta-Like DOCK6 : Dedicator Of Cytokinesis 6 DSHB: Developmental Studies Hybridoma Bank dx : deltex E(spl)-C ...
Fly strains were also obtained from the Vienna Drosophila Resource Center. The Ncc69 and fray cDNAs were obtained from the Drosophila Genomics Resource Center. Johannes Bischof and Konrad Basler generously provided the pUASg.attB plasmid. We thank the TRiP project at Harvard Medical School for ...
We thank the Bloomington Drosophila Stock Center (NIH P40OD018537) for providing fly stocks, the Drosophila Genomics Resource Center (NIH 2P40OD010949) for genomic reagents, and Flybase (NIH 5U41HG000739). We also thank the Indiana University Light Microscopy Center (LMIC) and the Indiana Un...
Dm Roquin cDNA was purchased from the Drosophila Genomics Resource Center, amplified by PCR and inserted into the HindIII and NotI restriction sites of the pAc5.1-lN-HA or pAc5.1-GFP vectors. For expression in HEK293T cells, the cDNA encoding Dm Roquin was inserted into the NotI and ApaI ...