Drop-seq_tools配合Picardtools,samtools STAR等工具实现对fq bam sam 等文件处理。流程首先将构建参考基因组文件夹,对物种的参考基因组fa及gtf文件做处理。 ● fasta:Thereference sequence of the organism.Neededformost aligners.● dict:Adictionary fileasgenerated by Picard’s CreateSequenceDictionary.N...
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Java and R tools for analyzing Drop-seq data Drop-seq questions may be directed to dropseq@gmail.com. You may also use this address to be added to the Drop-seq Google group. See Releases to download binaries. See Drop-seq alignment cookbook for detailed usage of these tools. See Census...
Drop-seq_tools配合Picardtools，samtools STAR等工具实现对fq bam sam 等文件处理。流程首先将构建参考基因组文件夹，对物种的参考基因组fa及gtf文件做处理。然后用Picard FastqToSam将下机文件转换成sam/bam 随后就可以利用drop-seq 的工具对bam文件做进一步的处理包括：Example Cell Bar...
Then, execute this command to build meta data that Drop-seq pipeline needs: ./build_meta.sh <reference_name> Put your raw data (formatted as <sample_name>_[1-2].fastq.gz or <sample_name>.bam ) in data/<sample_name>/ . If you start with FASTQ, you need to first convert the ...