giant viruses can be partitioned into six orders, 32 families, and 344 genera, substantially expanding our knowledge of the phylogenetic diversity of giant viruses [14]. Note, however, that in the abovementioned studies, most of the giant viruses (> 99%) were from marine and freshwater env...
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could be significantly distinguished (p-value < 0.01,R2 = 0.63) based on soil nutrient composition, soil pH, vegetation cover, and precipitation (Fig.2A, Figure S2). These results reflect the distribution of distinct biomes across sub-Saharan African countries, which are themselves define...
DNA was extracted using the PowerWater kit (QIAGEN, Germany) according to the manufacturer’s instructions, and quantified using Quantus (Promega, Madison, WI). 16S and 18S rRNA gene fragments were amplified using primers 515F–926R [38] and 528iF–964iR [29] respectively. Libraries were pre...
FaDu, Cal27, NCI-H1975 and Detroit 562 cells were obtained from ATCC. SNU1076 cells were obtained from the Korean Cell Line Bank. Cell lines were authenticated by short tandem repeat profiling at ATCC (Promega, Madison, WI, USA). All experiments were conducted with low-passage cell cultures...
P.O. Box 44283 Madison, WI 53744-4283 USA Tel:608-831-3433 Fax:608-831-3771 madisound.com info@madisound.com audioXpress May 2002 21 drivers in the prototype enclosure of Fig. 1A and made acoustic and electri- cal measurements on them. The mea- surements include acoustic frequency and...
The epidermal growth factor receptor (EGFR) is a clinically validated target in head and neck squamous cell carcinoma (HNSCC), where EGFR-blocking antibodies are approved for first-line treatment. However, as with other targeted therapies, intrinsic/acqu
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Punch biopsies were then taken from the corresponding FFPE tissue block and DNA was isolated using the Maxwell 16 FFPE Tissue LEV DNA Purification Kit (Promega, Madison, WI, USA). For detection of low frequency mutations with the Oncomine™ Colon cfDNA assay (see below), DNA was isolated ...