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The core components of CRISPR-based gene drives, Cas9 and guide RNA (gRNA), either can be linked within a self-contained single cassette (full gene-drive, fGD) or be provided in two separate elements (split gene-drive, sGD), the latter offering greater control options. We previously enginee...
Apoptosis analysis was then performed using FACS Canto II flow cytometry (BD Biosciences, San Jose, CA, USA). 2.9. Cell proliferation assay Cell proliferation was assessed using the Cell Counting Kit-8 (CCK-8) kit (YEASEN, Shanghai, China). A total of 3 × 103 cells were plated in 96...