Chloroform (assay ⩾99.5%, catalogue No. C2432) was purchased from Sigma–Aldrich, and used for dissolving DPPC powder. The water used in all experiments was purified by a Millipore system. The purified water had a resistivity of 18.2 MΩ cm at 25 °C, and the total organic carbon ...
(Cho1)均购于 Sigma化 学试剂公司,分子式如图 1所示.荧光剂 TexasRed(1,2-dihexadecanoyl-s凡一glycero一3一phosphoethano— laminetriethylammoniumsalt,TR-DHPE)购于 Invitrogen公司.其它试剂均为分析纯.水为离子交换三 次蒸馏水. (C) HO Fig.1 MolecularstructuresofDOPC(A),DPPC(B)andCholesterol(C) 1.2...
1999). The molecular weight of used enzyme PLC from Bacillus cereus amounts 23 kDa and contains 2 tightly bound Zn2+ cations whose optimal pH range is 6.6–8.0 (Sigma Product Information). The detailed study of Bjorklid and Little (1980) showed that the isoelectric point of the native ...
neutron reflectometry; ellipsometry; DPPC; lipid monolayers; air/water interface1. Introduction Synthetic in vitro lipid mono- and bilayers, as well as uni- and multi-lamellar vesicles, can be considered as simple biomembrane models. For more than 20 years, 1,2-dipalmitoyl-sn-glycerol-3-...
Figure 1. Chemical structure of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), a natural occurring bolalipid of the caldarchaeol-type, the single-chain model lipid PC-C32-PC, and the new glycerol diether bolalipid Me2PE-Gly(2C16)C32-OH investigated in this work. However, the main...