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First, prolonged neoadjuvant treatment is routinely applied (FOLFIRINOX or nab-paclitaxel plus gemcitabine, up to 8 cycles) at JHH and UPMC to assess both tumor biology (aggressiveness) and patient fitness. Second, although some multivisceral resections were nec- essary during the operation, ...
Murine Wnt1a cDNA (NM_021279, oriGene, MD, USA) was inserted into the Mlu I and Sal I sites of a retrovirus vector (PWPT, gift from Xin Wang, Shanghai Institute of Biochemistry and Cell Biology). Recombinant vectors were amplified in HEK293FT cells to generate high-titer preparations ...
The novel dPACS assay developed in this study will benefit the many specialities that use PCR-RFLP and other procedures for analysing SNPs and DIPs. It is a relatively inexpensive assay requiring basic equipment and consumables commonly available in all molecular biology laboratories. The dPACS ...