The retentate was then concentrated to a final volume of about 0.20 L and then filtered using a 0.45 μm sterile filtration unit (Corning, Corning, N.Y.). The filtered material was loaded at 7.5 mL/min onto a Pharmacia HR16/10 column which had been packed with PerSeptive Biosystem POROS...
Corning 公司.E-cadherin 抗体,N-cadherin 抗体和 GAPDH 抗体购自美国 Abcam 公司. 临床上对 TC 患者的治疗有肿瘤切除,药物靶向治 1.3 方法 疗,化疗放疗等手段,但治疗过程中仍然存在预后 差等问题 [2],对患者的健康和生活质量产生严重影 1.3.1 实时定量逆转录聚合酶链反应(qRT- PCR):用 Trizol 试剂提取组织...