Big, bustling sports bar with a college party vibe and a rooftop patio. Join us at The Double U.
Madison, Nebraska March 28, 2014 Overall Rating: My first attraction to this trailer company was their innovative 'SafeTack' design. As I started working with them I found that they were very knowledgeable about trailers and hauling our beloved equ Read Review Double D Trailer Review posted ...
GST fusion proteins were expressed in Escherichia coli Rosetta 2 (Novagen, Madison, WI) via incubation of the cells with 0.1 mm isopropyl 1-thio-β-d-galactopyranoside overnight at 15 °C and then prepared using His tag Binding/Wash Buffer (20 mm Tris-HCl (pH 8.0), 600 mm NaCl, 1 mm...
Cells were harvested and luciferase activity was measured using the dual luciferase reporter assay system (Promega, Madison, WI). Subcellular fractionation Subcellular fractionation were prepared using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific, Rockford, IL), according to the ...
Cells were also cotransfected with 0.05 μg/ml either p125-Luc or pISRE-Luc plasmid, and pSV-β-galactosidase vector (Promega, Madison, WI). After 24 hr, cells were stimulated with pIpC or LPS for 6 hr, and thereafter, cell extracts were prepared. The luciferase activity was measured ...
Bisulfite-modified DNA was recovered using a Wizard DNA clean-up kit (Promega, Madison, WI, USA) and desulfonated before PCR amplification. For COBRA-L1, bisulfate-treated DNA was subjected to 35 PCR cycles with two primers, B-L1-inward 5'-CGTAAGGGGTTAGGGAGTTTTT-3' and B-L1-outward 5...
Cell viability was determined after incubation of dispersed CNTs for 16 h as indicated by the CellTiter-Glo™ Luminescent Cell Viability Assay (Promega, Madison, WI). The effect of CNTs on cell proliferation was calculated as the percentage of inhibition of cell growth with respect to the ...
For control cells, the Pcmv promoter was replaced with the polyA-RNAP II transcriptional pause site from pGL3Basic (Promega, Madison, WI, USA), to block incoming transcription towards the lacZ gene. The cloning was done by ligation of the [BglII, Klenow-NheI] vector portion of pcDNA3.1-...
After washing twice with phosphate-buffered saline, the PC12 cells were fixed, incubated with anti-T7 tag mouse monoclonal antibody (1/5000 dilution; Novagen, Madison, WI) and anti-p300 rabbit polyclonal antibody (1/500 dilution; Santa Cruz Biotechnology, Santa Cruz, CA), and then visualized...
Department of Population Health Sciences, University of Wisconsin-Madison, Madison, WI 53726, USA * Author to whom correspondence should be addressed. Int. J. Environ. Res. Public Health2021,18(24), 13339;https://doi.org/10.3390/ijerph182413339 ...