We describe a novel rapid double histochemical staining method for in situ hybridization (ISH) and immunohistochemistry (IHC) of formalin fixed, paraffin embedded tissue sections. For this purpose, 3 cases of Epstein-Barr virus (EBV) positive gastric carcinoma tissues and 5 cases each of pulmonary...
Double Immunohistochemistry. 免疫组织化学 Labon... Preface: Youshouldnotco-staininIHCantigenslocatedonthesamestructure(nucleus,membrane,cytoplasm).Thatbecausethesuccessofthisdoublestainingprocedureisbasedonthecompletedevelopmentofthefirststain,whichshouldmaskthestructurestainedfirst.Thereforeyoushouldbeabletousetwoant...
Immunohistochemical double staining with estrogen receptor (ER) and epidermal growth factor receptor 2 (HER2) was conducted in tissue sample of 125 women with invasive breast cancer. The age at the time of surgery ranged from 28 to 82 years. The tumor size was 2 cm or less in 42 patients ...
IHC staining showed a thin dermis layer and a deficiency of CD163+ and CD206+ cells around the wound bed in the m-Clo‒treated group (Figure 9h). In addition, the extracellular matrix remodeling protein, α-smooth muscle actin as well as Pref-1+ cells were markedly increased in the ...
4) double immunohistochemical staining 免疫组织化学双重标记5) Immunohistochemistry 免疫组织化学染色 1. Methods: Ninety-three cases of Xinjiang Uygur with NHL were reviewed by pathologists to identified the diagnosis and subtype,combining the results of immunohistochemistry(IHC) stain. 方法:收集93例...
Double-labelling utilized a sequential IHC staining method, incorporating a “cocktail” of CD34, CD45 and CD68 as the first antibody visualized with the Bond Polymer Red Refine detection system (supplied by Leica Microsystems, Newcastle-upon-Tyne, UK) and MGMT clone 3.1 as the second antibody....
(B) IHC showed the representative results of low and high PDK1 expression based on the staining index on a tissue microarray. (C) Nude mice were subcutaneously injected with 8 × 106 HCT116 cells with or without the transduction of PDK1 shRNA. Silencing PDK1 significantly slowed down the...
The IHC scoring of specimens was determined by the staining intensity and extent scores, which graded as 0 (0%), 1 (1–25%), 2 (26–50%), 3 (51–75%), or 4 (76–100%). Antibodies used were shown in Table S1. Plasmid construction, lentivirus infection, and transfection shRNA ...
IHC staining of the MMR protein revealed lack of expression of the hMSH2 protein, whereas hMSH6 was normally expressed (data not shown). The patient was treated with surgery and no drug therapy. In June 2022, the patient was diagnosed with endometrioid adenocarcinoma. Surgery pathological showed...
(c, g) Tumor tissue was harvested 24 h after completion of treatment, H&E staining done to visualize morphology, and immunostaining done to detect the proliferation marker Ki67. Scale bar = 10 μm. (d, h) IHC data for Ki67 were quantitated as the number of Ki67 positive tumor...