One of the notable examples of environmental flow is the estimation of groundwater flow, which is critical for tunnelling construction [4], groundwater extraction [5,6], and contamination prediction [7]. A massive number of works on the numerical simulation of groundwater flow have been ...
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Plasmid construction and transfection. Plasmids used in this study comprise a tet-on inducible system and a regulated mammalian coumermycin inducible expression system. EGFP-N1 (Clonetech, USA) was used as a control. The tet-on inducible system was as previously described20. Sox9 expression ...
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