Fig. 2. OD from plasma samples tested in EIA including all three antibodies (All ab.), EIA with secondary antibody substituted with dilution buffer only (No sec.ab.), and EIA with tertiary antibody substituted with dilution buffer only (No tert. ab.).V. VIDEM...
Always wash hands after removing gloves.Incompatibilities Avoid prolonged contact with any solution of an alkaline pH as it will result in hydrolysis of the drug. Do not mix Vero-Epirubicin with heparin or fluorouracil due to chemical incompatibility that may lead to precipitation....
We also investigated the in vivo biodistribution and clearance of EA in tumor-bearing mice. 2. Materials and methods 2.1. Chemicals Sodium erythorbate monohydrate (EA-Na·H2O) was obtained from Tokyo Chemical Industries (Tokyo, Japan). Sodium ascorbate (AA-Na), heparin, calcein-AM solution and...
In order to evaluate effects of PERM on bioenergetics, brain mitochondria were isolated and O2 consumption was measured. 2.3. Permethrin Oral Exposure PERM was dissolved in corn oil and administered orally (n = 6) for 15 days, at daily doses of 150 mg/kg body weight per day (1/10 of ...
A potential mechanism for these secondary elevations is discussed later in this review (see Figure 5). 3. History of GAG Assay by Tandem Mass Spectrometry (MS/MS) Initial MS methods for GAG assays were developed to detect heparin oligosaccharide mixtures with electrospray mass spectrometry (EMS) ...
Whole blood was collected from all donors in heparin-coated tubes. Peripheral blood mononuclear cells (PBMC) were isolated by density-gradient sedimentation using Ficoll-Paque (Euroclone). The plasma was then carefully removed from the cell pellet and stored at −20 °C to evaluate the level o...