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La trama d'error consisteix en dos camps diferents: El primer camp ve donat per la superposició de l'ERROR FLAG (6-12 bits dominants/recissius) contribuïts de diferents nodes. 错误帧由两个不同的字段组成: 第一段由不同站点提供的错误标志(6-12个显性位/隐性位)的叠加给出。 LASER-...
But there is a critical error in one of your two copies of that gene. Which of the two copies will your cells use? Answer: Both! There is no flag as I speculated above. So when your cells manufacture some quantity of the protein, about half of those protein molecules are defective, ...
Clone RAW-MB01-WT3.1 was used in the present study. Expression of flag-TTP was found to be strongly inducible by LPS but only weakly responsive to tetracycline, because of leakiness of the tetracycline-regulated promoter in RAW264.7 cells (data not shown)....
pUAST-Yki ΔC:Flag(3×) was created using HiFi Assembly (NEB) and lacks the last 7 residues of Yki (sequence LEWYKIN). pLL3.7-EF-EYFP-YAP1_delta5C-PolyA (Addgene #112289). pLL3.7-EF-EYFP-YAP1_WT-PolyA (Addgene #112284). pLL3.7-EF-EYFP-YAP1_ΔRK2 contains these mutations: ...
SpCas9-DN2 and SpCas9-DN2L. The transfected cells were sorted for GFP+ cells and lysed for western blotting as described above. Primary antibodies used were to FLAG (1:1000; Sigma F1804) and actin (1:1000; Santa Cruz sc-8432). To assess the stability of Cas9-DN1S with and without...
The top half of the image is the previous command which runs in Lab mode by default. The bottom half of the image was the previous command passed with the addition of the--rgbflag. Passing the same colors without--replaceresults in the image below, which colors the pixels with the closes...
Means and error bars of the percentage Jurkat cells death are shown. Data represent two independent duplicate experiments. (c) Surface staining of FLAG-tagged FasL in 293T cells transfected with empty vector, hgld-, WT-, and Pt 86- FasL as indicated. Full size image Interestingly, FasL ...
(B) CARD11-KO Jurkat T cells reconstituted with myc-WT-FLAGx2 or myc-mutant-FLAGx2 were stimulated with PMA/ionomycin for 30 min, followed by the denatured co-immunoprecipitation assay with rabbit anti-Bcl10 and Western blotting for linear ubiquitin and Bcl10. (C) Reconstituted cells were ...
We used HeLa cells to generate a cell line with stable Dox-inducible expression of FLAG-tagged Opa11–289 (Figure 5A). (The breakpoint at residue 289 had been chosen for an earlier study, but is very close to the predicted protein truncated at residue 285 in the mutant mice; both would...