Briefly, naïve male mice were culled by overdose of sodium pentobarbitone (0.4 ml) while LPS-treated female mice were eu- thanized with 0.4 ml of Avertin (2.5%) one day after final LPS administration. The trachea was cannulated (20 G Intima, BD Scientific) for infl...
the BRCA2 F15X peptides as orange (chain E) and red (chain F) tubes. A zoom view of the dimerization interface between ARM chains A and D is displayed in a dotted box: only helices α1–α4 are displayed for clarity. The dimerization interface is mediated by hydrophobic residues from ...
After the muscle had been homogenized and optimally diluted, 4–5 drops were applied to cold coverslips, incubated for 20 min, fixed 10 min in fresh 4% paraformaldehyde in PBS, and stored at 4°C in PBS + 0.02% sodium azide. Immunofluorescence and microscopy For isolated myofibrils, ...
Note: A lens’saperturerefers to a hole in the lens, which opens and closes depending on your camera settings. A wide aperture has certain special effects, and these are only offered by fast lenses. Now, wide apertures correspond to low f-numbers, such as f/1.4, f/1.8, and f/2.8. ...
Hormel Spam. – This cheap processed meat product might not be a gourmet delight, but it is filled with protein, sodium, and “good” fats that should help your body strong during a long-term disaster. You could mix the canned Spam into soup or stew recipes to create more filling and ...