light3]The light microscope cannot resolve detail finer than 0.2 micrometers, about the size of the smallest bacterium; consequently, no matter how many times its image of such a bacterium is magnified, the light microscope cannot show the details of the cell’s internal structure.[/highlight3...
A simple microscope has one lens and is essentially a loupe or magnifying glass with a relatively high magnification. The basic modern microscope found in schools, hospitals, and research centers is a compound microscope which has a series of lenses to collect and focus the light transmitted throu...
To determine the amount of fibrosis, 5 pictures of the Sirius Red stained sections per LV were taken with a light microscope (20x magnification). From these pictures, the Sirius Red positive area was automatically calculated as a percentage of the total tissue area using an in house made ...
where the Y chromosome is one of the smallest chromosomes, it represents less than 2% of the genome. It is possible that animals have evolved to express heterochromatin factors at levels that can mitigate
Fluorescence images were obtained using an iMIC confocal microscope (TILL Photonics, FEI, Germany) equipped with the following objectives: ×10, numerical aperture (NA) = 0.35; ×20, NA = 0.75; ×40, NA = 1.3; and ×60, NA = 1.46 as well as appropriate filter settings for detection of...
A microscope at high magnification reveals a closer look at the diversity of sand. This sample of sand from Sand Beach in Maine collected by Robert Maronpot includes a white sea urchin spine to the left of a large striped tan mollusk shard. Some clear quartz sand grains are just to the ...
Images were acquired using a Leica TCS SP8 confocal microscope. Quantitative analysis of lymphatic vessel growth was performed using Fiji software. Area coverage was calculated for each sample in a field of view centered on the confluence of sinuses: (area of LYVE1 signal/area of field of view...
Metastatic potential was measured by the presence of phosphorylated myosin light chain (MLC2). Tumour microarrays were examined immunohistochemically for UCH-L1 expression. Kaplan-Meier curves were generated using UCH-L1 expression levels and patient survival data extracted from Gene Expression Omnibus ...
RESULTS Light microscopy. Figure 2A and C show representative pictures of an early pubertal female growth plate (patient 4) at 40ϫ (A) and 100ϫ (C) magnification. Figure 2B and D show pictures of the late pubertal patient (patient 13) with ongoing fusion of her growth plate stained ...
microscope with attached Canon EOS 100D camera at × 19.4 magnification. All specimens were then rotated 90° laterally, and imaged whilst propped onto their penultimate chamber. Images were processed using the image analysis software Image Pro Premier, and the “size” trait parameters: test ...