Unlike isopropanol, hydrogen peroxide is not a type of alcohol. You might recognize its chemical formula, H2O2, as being similar to that of water (H2O). The difference is thathydrogen peroxide has two oxygen atoms instead of one. That one extra oxygen atom makes it a strong oxidizer. How ...
Briefly, 50-100 mg frozen tissue was pulse homogenised in 1 ml TRI Reagent (Sigma, UK), and tissue homogenates incubated at room temperature for 5 min to allow for nucleoprotein complexes to completely dissociate from RNA. Chloroform was added to homogenates (200 μl/1 ml TRI Reagent), ...