Recent studies have shown that ultra-high pressure homogenization (UHPH) improved the techno-functional properties of the granule, but they did not evaluate its impact on digestibility. Consequently, this work aimed to evaluate the impact of UHPH-treatment (300 MPa and 4 passes) on granule ...
CavitationDroplet breakupHigh-pressure homogenizationOrificeHigh-pressure homogenization is a popular method to produce emulsions of droplet sizes smaller than 1 m. Regarding the role of cavitation in the process, it is still discussed controversially whether it promotes or hinders droplet breakup during ...
Whole homogenates of HEK cells were made in homogenization buffer including 50 mM PIPES pH 7.2, 10% glycerol, protease inhibitor cocktail, and 1 mM DTT. The homogenates were cleared by centrifugation at 14,000 RPM at 4 °C for 20 min. For all expression experiments, cells were harvested 24...
Erythropoietin (Epo) exerts direct effects on white adipose tissue (WAT) in mice in addition to its erythropoietic effects, and in humans Epo increases resting energy expenditure and affect serum lipid levels, but direct effects of Epo in human WAT have not been documented. We therefore investigat...
Cells were lysed by homogenization on ice for 3 min. The pro- tein concentration was determined using the Bio-Rad protein assay (Bio-Rad Laboratories, Hercules, CA, USA). Samples were mixed with Laemmli buffer and denatured at 100 ºC for 5 min, and then 40 µg of total protein ...
If you are injecting cows with unnatural levels of hormones, it is obviously going to negatively affect not only the cow itself, but you, the consumer who is drinking that cow's milk or eating any of it's byproducts. Modern technology is going too far trying to mass produce animal ...
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Bile acids serve a vital function in lipid digestion and absorption; however, their accumulation can precipitate liver damage. In our study, we probed the effects of dimethyl sulfoxide (DMSO) on bile acid synthesis and the ensuing liver damage in mice in
At the end of the homogenization process, a pool of lung homogenate was prepared mixing equal amount of homogenate from three different donors. Test compounds and the positive control were diluted in the pooled lung homogenate to a final concentration of 5 mM and then dispensed into the red ...