The fatty acids were extracted with 1 ml of chloroform. The extraction was repeated three times, and the extracts were pooled and dried in a steam of nitrogen. Methylation of the carboxyl groups was carried out using diazomethane in ether (0.1 ml), freshly prepared using 1-methyl-3-nitro-...
view, the aniline part of DAMBA (a derivative of DA; vide infra) could be easily coupled with salicylaldehyde to yield Schiff bases, which usually owns flexible structure with photoisomerization property42,43,44,45. While the alicyclic (decalin) structure of DAMBA actually could be thought as ...
Plasma glucose was first extracted with chloroform–methanol–water and derivatization was performed with butylboronic acid and acetic anhydride as described previously [27]. After derivatization, plasma [6,6-2H2] glucose enrichment was determined by electron ionization GC-MS (Finnigan INCOS-XL). ...
The reaction products were phenol/chloroform extracted and ethanol precipitated using pellet paint (Novagen) plus tRNA. The purified DNA was then digested with the Sfc I restriction endonuclease (NEB) for 2 h at 25°C followed by labeling with [α-32P]-TTP for 2 h ...
Briefly, lipid solutions in chloroform were dried under a stream of N2 and residual organic solvent was removed in vacuo (16 h). The lipid film was hydrated with the desired buffer and subjected to eight freeze-thaw cycles to produce multilamellar vesicles (MLVs). The MLVs were extruded ...
Theade1-14allele, which is used for [PSI+] selection, is actually located on chromosome I. The presence of an additional chromosome I could therefore constitute a selective advantage in absence of adenine, as increasedade1-14mRNA would lead to more Ade1. To test this idea, we generated a...