(F2442, Sigma-Aldrich) in dPBS before FACS purification. This was done for samples collected from four different fish that were processed in two different cycles, combining skin samples from two different fish for each cycle. The flow cytometry experiments were performed on a BD FACSAria II ...
Airway cells were either stained for flow cytometry or the cellular composition was determined by spinning 1–2 × 105 cells onto a microscope slide (Thermo Scientific) at 450 rpm for 5 min using Cytospin 4 Cytocentrifuge (Thermo Fisher Scientific). Slides were H&E stained using Rea...