Five indexed 96-well plates were collected for each skin band. After sorting, the samples were spun down at 3166 r.c.f. at 4 °C for 2 min in an Eppendorf tabletop 5810 R centrifuge (Eppendorf AG, Hamburg, Germany) and stored at −80 °C until further processing. scRNA...
To pellet PEVs, the supernatant was carefully transferred to another tube and centrifuged 22,000× g for 45 min [58]. Subsequently, the pellet was aliquoted in dPBS and frozen at −80 °C until use. All centrifuge steps were made at 4 °C. Extracellular vesicle protein concentration was...
15-mL centrifuge tubes to 1.0-mL microtubes. New 35- and 50-micron sample line filters minimize clogging during a sort. Sort collection chamber The new universal mount in the sort collection chamber makes inserting the tube holders easier. ...