Extraction of Nicotiana tabacum cell cultures, chromatography on DEAE-cellulose and gel filtration resulted in a homogeneous protein (Mr= 14500), which strongly reduces the hydrolysis of Escherichia coli DNA by DNase I. DNA degradation by micrococcal nuclease is not inhibited. The inhibitor protein ...
我要询价 添加 PBM HapCult™ 温和细胞解离试剂/Cell dissociation reagent 产品类型:细胞消化酶 规格:100mL 608010 我要询价 添加 PBM 0.5mM EDTA,pH 7.0-7.2 产品类型:细胞消化酶 规格:100 mL 205010 我要询价 添加0.5mM EDTA,pH 7.0-7.2 Anti-Biotin positive selection kit/阳性磁珠分选试剂盒 纯度高活...
The cells were subjected to lysis by sonication and cell debris was removed by centrifugation at 23,708 × g for 40 min at 4 °C. The lysate was first purified using Ni2+-NTA resin. The beads were washed and the bound proteins were eluted by buffer B (25 mM Tris-HCl, ...
The space group of I212121 did not yield any solutions. This model gave an initial R value of 51.6% for data between 8 Å and 3.2 Å resolution. Subsequent rigid-body refinement using all data between 40 Å and 2.3 Å gave an R value of 46.2%. In the unit cell, Im7 ...
摘要: Presents a kinetic and thermodynamic analysis of complex formation for a secreted bacterial toxin colicin E9, and an isolated DNase domain within the toxin, with the immunity protein Im9. Calculation of the dissociation constant; Two-step binding mechanism; Temperature dependence of equilibrium....
Of course, mutations that disrupt the toxin/immunity protein complex should be lethal to the cell; thus, the pressure to retain high-affinity interactions is presumably a significant barrier to diversification. However, colicin/immunity protein complexes have some of the highest known binding affinities...
Cell-2016-PAM-Dependent Target DNA Recognition and Cleavage by C2c1 CRISPR-Cas Endonuclease 热度: Pressure dissociation of integration host factor-DNA complexes reveals flexibility-dependent structural variation at the protein-DNA interface 热度:
in subdomain 2 of actin on F-actin dynamics were investigated by measuring the rates of the polymer subunit exchange with the monomer pool at steady state and of ATP hydrolysis associated with it, and by determination of relative rate constants for monomer addition to and dissociation from the ...
Briefly, the cells were detached from the plate with Cell Dissociation Solution (Sigma-Aldrich, Darmstadt, Germany), washed with PBS and resuspended in 200 µL of 1× binding buffer supplemented with 2 µL of Annexin V-FITC and 2 µL of propidium iodide (for apoptosis analysis) or 2...
A specific protein complex containing ATPase has been identified; this enzyme powers lipopolysaccharide transport from the cytoplasmic membrane across the cell envelope [39]. ATPase with high activity was found in a highly stable multiprotein complex of the female placenta [11]. With this in mind,...