RNase Activity Assay Kit (Fluorescence)(Cat. No. ASE-A001) 在Qubit(左图)、qPCR(中图)和酶标仪(右图)平台上检测DNase的标准曲线 在Qubit 荧光仪、qPCR和酶标仪上分别检测3个已知浓度的DNase质控品,回收率在70%-130%范围内,说明在不同仪器上均可进行准确测定。 高灵敏度:在酶标仪、qPCR仪和Qubit荧光仪检...
The DNase Activity Assay Kit (Fluorescence) is based on a fluorophore-labeled DNase substrate. When the sample does not contain DNase activity, the substrate is stable and does not produce a fluorescent signal; when the sample contains DNase activity, the substrate is degraded, resulting in a gr...
碧云天研发的DNase活性荧光检测试剂盒(DNase Activity Fluorometric Assay Kit, or Fluorometric DNase Assay Kit),是一种用荧光法快速高灵敏检测DNase I及其它脱氧核糖核酸酶(Deoxyribonuclease, DNase)活性的试剂盒。 脱氧核糖核酸酶和核糖核酸酶(RNase)类似,广泛存在于实验环境和生物物体中,由于核酸酶能够降解核酸,因此...
//www.beyotime.com DNase活性荧光检测试剂盒 产品编号 P0345S P0345M 产品名称 DNase活性荧光检测试剂盒 DNase活性荧光检测试剂盒 包装 100次 500次 产品简介: 碧云天研发的DNase活性荧光检测试剂盒(DNase Activity Fluorometric Assay Kit, or Fluorometric DNase Assay Kit),是 一种用荧光法快速高灵敏检测...
Updated procedures measure DNase I activity using real-time fluorescence assays which allows for fast, quantitative measurements. Optimal Digestion Conditions Like many enzymes, DNase I activity is affected by the composition of the reaction buffer. Contrary to popular belief, DNase...
The activity assay was based on previous work, with slight modifications18. The WT or mutant purified DNase II protein (10–50 ng) was incubated with 500 ng plasmid DNA in the activity reaction buffer (10 mM Tris/HCl pH 5.3, 5 mM EDTA pH 8.0). The protein/DNA mixture was incubated ...
Top I-mediated ligation assay was carried out using the ApopTag ISOL Dual Fluorescence Apoptosis Detection Kit (DNase Types I & II) (Millipore, Billerica, MA, USA). Fixed embryos were rehydrated, washed with PBTw, incubated in the ISOL labeling reaction mixture in a dark humid chamber for ...
Fluorescence-based nuclease activity assays, in which the DNA substrate is labelled with PicoGreen dye, are cheaper, safer, and easier to perform. Herein, the results of the fluorescence-based nuclease activity assay were consistent with the results of the two conventional methods. In addition, th...
Moreover, our cleavage assay using 2’-deoxy-substituted ssRNAs at nucleic acids 6, 12, 18, 24, and 30 also reveals that the cleavage activity is largely abrogated by the ssRNA carrying this mutation at 24 (Supplementary information, Fig. S8c and d), further supporting the notion that ...
(B) In vitro DNase activity assay. The integrity of plasmid DNA (pTrc200) coincubated with purified proteins of the wild-type 4350 (WT) or the H190A D193A catalytic site mutant in the presence (+) or absence (−) of Mg2+ at 37°C for 1 hr was visualized with 1% agarose gel. ...