RNA–DNA hybridsS9.6 antibodySpike-inqDRIP (quantitative DNA–RNA immunoprecipitation)R-loops are three-stranded nucleic acid structures, comprising an RNA-DNA hybrid and a displaced strand of ssDNA. R-loops have important physiological roles in cells, but deregulation of R-loop dynamics can also ...
基于抗体的DNA修饰位点鉴定是一种传统的，被广泛应用的较为简单的方法。目前5mC、5hmC、5fC、5caC均有商业化的抗体，可用于做DNA IP(DNA immunoprecipitation, DIP)。利用5hmC-DIP可鉴定癌症患者血液中循环DNA的5hmC【22】，说明此方法灵敏度高，无须耗费大量DNA，具有临床应用的潜力。但是DIP无法提供单碱基精度的...
5. Chromatin Immunoprecipitation(染色质免疫沉淀) 染色质免疫沉淀(ChIP)是一种基于抗体与特定蛋白质结合的技术,可以捕获与该蛋白质结合的染色质。ChIP-seq是一种基于ChIP的技术,通过使抗体附着在特定蛋白质上,捕获与其结合的DNA片段。通过这种方法,可以确定特定蛋白质与染色质结合的位置和强度,从而揭示DNA-RNA相互作用...
Immunoprecipitation of DNA:RNA Hybrids Using the S9.6 Antibody. PMID: 32681514Catalytically inactive, purified RNase H1: A specific and sensitive probe for RNA-DNA hybrid imaging. PMID: 34232287Mapping R-Loops and RNA:DNA Hybrids with S9.6-Based Immunoprecipitation Methods. PMID: 34515688...
LncRNA HOTAIR 高表达,通过与AR直接结合,抑制了E3泛素酶MDM2介导的AR降解,从而上调AR表达水平,并以雄激素非依赖的方式激活AR下游基因转录表达,促进CRPC细胞生长和侵袭。 在介绍全文以前,我们先科普几个技术,IP,RIP,RNA Pulldown和CHIP。 IP,immunoprecipitation,免疫共沉淀,检测蛋白 ...
[32] Mohn F, Weber M, Schübeler D, Roloff T-C. Methylated DNA Immunoprecipitation (MeDIP), 2009, p. 55–64. https://doi.org/10.1007/978-1-59745-522-0_5. [33] Dominissini D, Moshitch-Moshkovitz S, Salmon-Divon M, Amariglio N, Rechavi G. Transcriptome-wide mapping of N6-...
Fluorescence in situ hybridization, FISH）和染色质免疫共沉淀（Chromatin immunoprecipitation assay kit, ...
5. Ago HITS-CLIP (high-throughput sequencing of RNAs isolated by crosslinking immunoprecipitation) 前面已经提到miRNA可以与mRNA结合调控基因的表达,有研究指出miRNA仅需要6-8nt与mRNA精确配对即可发挥作用。但这对利用碱基组成重头预测靶标 mRNA是一个巨大的挑战。miRNA与Argonaute蛋白复合物构成RISC核心元件,并引导...
They observe such hybrids by developing a purification method named cytoplasmic DNA–RNA hybrid immunoprecipitation (cytoDRIP). Notably, the formation of cytoRDHs depends on both the endonuclease XPG, which is known to process nuclear R-loops, and exportin-1, which actively exports nucleic acids to...
7.Sanz LA, Chédin F. High-resolution, strand-specific R-loop mapping via S9.6-based DNA-RNA immunoprecipitation and high-throughput sequencing.Nat Protoc.2019 Jun;14(6):1734-1755. 8.Graf M, Bonetti D, Lockhart A, Serhal K, Kellner V, Maicher A, Jolivet P, Teixeira MT, Luke B. Te...