GST pull down(GST融合蛋白沉降技术)是体外检测蛋白相互作用的常用方法,一般来说,GST融合蛋白pull down方法用于两个方面:一是鉴定能与已知融合蛋白相互作用的未知蛋白质;二是鉴定两个已知蛋白质之间是否存在相互作用。为排除假阳性所设的一组对照实验是在没有诱饵蛋白存在的条件下,将样品结合到GST上。该对照可以是表...
1.RNA Pull Down Assay(1) 为了鉴定特定的RNA分子是否与某一种特定的蛋白结合,研究人员根据DNA-Protein实验中DNA Pull down的实验方法进行改进,使其在RNA上实现。 技术步骤如下: (1)设计末端携带T7 RNA Promoter的引物扩增DNA片段; (2)利用T7 RNA polymerase 进行转录,转录过程添加携带生物素的dNTP,使转录本携...
1. GST Pull Down 基本原理是利用GST融合蛋白吸附细胞提取物中的配体蛋白。首先，通过基因工程将目标蛋白与GST融合，然后在含有GSH的色谱柱上纯化。实验中，通过添加GST融合蛋白和细胞裂解物，结合后通过洗脱和电泳分析，确认蛋白质间的相互作用。2. DNA Pull Down 这种技术用于研究DNA与蛋白的相互作用...
DNA-protein pull-down and Chromatin immunoprecipitation (ChIP) analyses of binding of nuclear proteins of TPA±BI treated H9 cells to TRE III.Ammar, AbouKandilRachel, ChamiasMahmoud, HuleihelW., T. GodbeyMordechai, Aboud
b, Dsk2 pulldown of ubiquitylated proteins from RPE1 cells synchronized in G1 by serum starvation and treated with UVC or FA, or from cells released from a thymidine block into S-phase in the presence of deoxycytidine (dC) or 5-aza-dC, representative of three independent experiments. c, ...
To identify which proteins are involved in PARP1-dependent DPC resolution, we performed plasmid pull-down mass spectrometry (PP-MS)9. To this end, a control plasmid (pCTRL) and pMHssDNAwere incubated in HSS in the presence or absence of PARGi and PARPi (Fig.3A). Plasmids and associated...
DNA-protein interactions can be studied bychromatin immunoprecipitation (ChIP). This technique involves the immunoprecipitation or pull down of DNA with antibodies specific for the DNA-associated proteins. Histone modifications, as well as transcription factors, enzymes, and othe...
Stringent plasmid pull-down was performed as in (A). RTEL1-Dependent DPC Bypass Promotes SPRTN Activity To examine whether RTEL1 is required for SPRTN activity, we examined meDPC, which is not susceptible to the proteasome but can be degraded by SPRTN (Larsen et al., 2018). The action ...
Pull-down experiments demonstrated that TFIIH binds to XPA·DNA complexes in an UV damage-dependent manner by a direct protein-protein interaction with XPA. We propose that an enhancement of the affinity of XPA protein for TFIIH could arise from conformational changes of XPA when it binds to...
Explore:KingFisher Flex System with Dynabeads reagents: A consistent approach for exosome pull-down and analysis Advantages of automated extraction and purification Reduces number of manual steps KingFisher instruments save you...