The RIPE3b1 DNA-binding factor plays a critical role in pancreatic islet β cell-specific and glucose-regulated transcription of the insulin gene. Recently it was shown that RIPE3b1 binding activity in β-cell nuclear extracts is reduced by treatment with either calf intestinal alkaline phosphatase...
Acetylation regulates transcription factor activity at multiple levels. CREB-binding protein (CBP) possesses an intrinsic acetyltransferase activity capable of acetylating nucleosomal histones as well as several nonhistone prot... E Soutoglou,N Katrakili,I Talianidis - 《Molecular Cell》 被引量: 763...
DNA sequence analysis revealed a binding motif for octamer-binding transcription factor 1 (OCT-1) in the deleted fragment. Mutation of this OCT-1 binding motif resulted in a similar effect as the deletion experiment, i.e. it increased the basal promoter activity and abolished the response to ...
DNA sequence analysis revealed a binding motif for octamer-binding transcription factor 1 (OCT-1) in the deleted fragment. Mutation of this OCT-1 binding motif resulted in a similar effect as the deletion experiment, i.e. it increased the basal promoter activity and abolished the response to ...
The redox regulation mechanism has previously been known for a number of transcription factors such as Jun/Fos (25, 30), Myb (31, 32), NF-κB (26), and Ets-1 (33). Their DNA binding activity is greatly stimulated by sulfhydryl protecting agents such as DTT and mercaptoethanol or by ce...
CCA1 and LHY areMyb transcription factors that bind directly to the TOC1 promoterto negatively regulate its expression. Conversely, the activity ofTOC1 has remained less well characterized. Genetic data supportthat TOC1 is necessary for the reactivation of CCA1/LHY, but thereis little description...
The combination of TNFα and GSNO produced a relative CAT activity of 3446 ± 383, which was significantly higher than that of GSNO alone (p < 0.05), indicating that transcriptional induction of IκBα by GSNO most likely occurred through transcription factor(s) other than NF-κB. We ...
occupancy taskdiscriminates genomic motif instances that are bound by a transcription factor (positive set) from motif instances that are not bound by the same transcription factor (negative set) in the same cell type, where GC-content and motif strength are matched between the positive and ...
The RIPE3b1 DNA binding factor plays a critical role in pancreatic islet β cell-specific and glucose-regulated transcription of the insulin gene. Recently it was shown that RIPE3b1 binding activity in β cell nuclear extracts is reduced by treatment with either calf intestinal alkaline phosphatase...
We have examined the activity of the Xenopus heat-shock transcription factor (HSF) in extracts from stressed and unstressed embryos at various stages of development using DNA mobility shift analysis. A specific interaction between HSF and a synthetic oligonucleotide corresponding to the proximal heat-sh...