More recently, DNA-conjugated antibodies have emerged as a promising technology, capitalizing on the programmability and amplification capabilities of DNA to enable highly multiplexed and ultrasensitive protein detection. However, the nonspecific binding of DNA-conjugated antibodies has impeded the widespread...
The barcodes are formed by in situ hybridization of tetrahedral DNA nanostructures and short DNA sequences conjugated with protein-targeting antibodies, and by nanostructure-assisted ligation (either enzymatic or chemical) of the nanostructures and exogenous DNA sequences bound to nanoparticles of ...
and the presence of target analytes (iii) was determined.b, Barcoded probe design. The probe consists of a Y-adapter containing the motor protein;
of barcodes per antibody allowing for more complementary probe strands to bind Amplification Detection Staining Single Antigen Retrieval Step Single Staining Step Single Amplification Step Single Detection Step Mixture of DNA- barcoded antibodies Mixture of fluorescent ...
Quantifying protein abundance on single cells using split-pool sequencing on DNA-barcoded antibodies for diagnostic applicationsProteins play critical roles across all facets of biology, with their abundance frequently used as markers of cell identity and state. The most popular method for detecting ...
of barcodes per antibody allowing for more complementary probe strands to bind Amplification Detection Staining Single Antigen Retrieval Step Single Staining Step Single Amplification Step Single Detection Step Mixture of DNA- barcoded antibodies Mixture of fl...
Antibodies Bioinformatics Immunological techniques Software This article is cited by Molecular and physiological changes in the SpaceX Inspiration4 civilian crew Christopher W. Jones Eliah G. Overbey Christopher E. Mason Nature (2024) Virome-wide detection of natural infection events and the associat...
The barcodes are formed by in situ hybridization of tetrahedral DNA nanostructures and short DNA sequences conjugated with protein-targeting antibodies, and by nanostructure-assisted ligation (either enzymatic or chemical) of the nanostructures and exogenous DNA sequences bound to nanoparticles of ...