More broadly, our work demonstrates how single-cell technologies can elucidate the complex interplay between DNA mutations and their effects on protein expression in cancer. Results To achieve combined DNA sequencing and antibody profiling in single cells, we developed a workflow leveraging a commercial...
A section of DNA containing instructions for making a specific protein. Genes determine many physical characteristics, such as eye color and height. Genetics:The scientific study of genes and heredity. Heredity:The passing of genetic character from parents to their offspring through genes. ...
However, if I packed and checked out, got to the airport, and my flight was canceled, I was likely going to be stranded, potentially at the airport for at least two days. There aren’t any hotels in the Salt Lake City airport, but there are a few nearby. However, probably not enoug...
Intrinsically disordered regions (IDRs) in DNA-associated proteins are known to influence gene regulation, but their distribution and cooperative functions in genome-wide regulatory programs remain poorly understood. Here we describe DisP-seq (disordered protein precipitation followed by DNA sequencing), an...
She told me to take the turkey out and eat it because I needed the protein. Bless her heart. She was right. I grabbed two forks in the food booth, and we both ate out of the fruit box positioned halfway between us. Then, after discussing and laughing that the muffin looked like a...
DNA methylation is one of the most commonly studied epigenetic marks, due to its role in disease and development. Illumina methylation arrays have been extensively used to measure methylation across the human genome. Methylation array analysis has primar
An optimized strategy incorporating RNA next generation sequencing to screen for gene fusions in colorectal carcinomas. CRC, colorecta carcinoma; NGS, next generation sequencing; MMR, mismatch repair Full size image Molecular genetic features of dMMR CRCs with gene fusions RNF43(17/22, 77%), FAT...
The secondary objective was to investigate genomic instability in the case of a MMR protein loss. Expression of proteins was assessed by using immunohistochemistry and microsatellite instability (MSI) performed by next generation sequencing. Nectin-4 expression was reported using an intensity scoring ...
Precise measurement of the full complement of genomic DSBs present in cells has been a major challenge to achieve at scale. This is because most physiological breaks are infrequent and occur stochastically throughout the genome. Existing sequencing-based methodologies typically use classic amplification-...
Seed size/weight is a major agronomic trait which determine crop productivity in legumes. To understand the genetic basis of seed size determination, we sought to identify DNA polymorphisms between two small (Himchana 1 and Pusa 362) and two large-seeded