The specificity of triplexes relies in the formation of Hoogsteen hydrogen bonds between the third-strand bases and the purine bases of the duplex (Rajagopal and Feigon, 1989b; Soyfer and Potaman, 1996; Thuong and Hélène, 1993). Triplexes can be classified based on the composition and ...
The resulting labeled molecule can be microscopically observed to determine the base sequence of the nucleic acid.doi:US5470707 AYuji SasakiYoshinori HaradaUSUS5470707 Mar 4, 1993 Nov 28, 1995 Hitachi, Ltd. Hydrogen bond labeling and base sequence determination methods for DNA or RNA...
DNA denaturation refers to the process in which the hydrogen bonds and van der Waals interactions that hold the double helix structure of DNA together are weakened, leading to the separation of the DNA strands. This can be induced by various agents such as heat, strong alkalis, urea, and for...
another important residues, Gln143. Consistently with previous observation, the high-resolution DUX4HD2-DNAERGstructure reveals an interesting Gln143-water-nucleotide network in the major groove (Fig.1f). Furthermore, via water-mediated hydrogen-bonding network, Gln143 can contribute to side-chain or...
(Supplementary Video6). In the Primer Separation structure, two terminal nucleotides in the 3′ end of the primer are found in the exonuclease channel (Fig.5c, f). The terminal base is inserted into theexosite and stabilized by hydrogen bonds with N270, D274, D198, and D399 residues (...
The short protein was successfully expressed, purified and used for crystallisation purposes in complex with Cy5-labeled DNA. The structure, which was obtained from blue crystals, shows a typical ring-shaped bacterial β-clamp formed of two monomers, each with three domains of identical topology, ...
The other carboxyl group forms hydrogen bonds with the amide proton of Lys68 (distance of 1,67 Å) and with the hydroxyl group of Thr67 (distance of 1,94 Å). Obviously, the two carboxyl groups contribute to pamoic acid affinity for the 8 kDa domain. Using one of them to tether ...
models. Stable hydrogen bonds, including model-specific ones, were well consistent across the four runs (Additional file1: Fig. S5B); the 90% cut-off of the mean occurrence identified as stable all Watson–Crick bonds and 79% of the main-chain bonds observed in the 1XC8 crystal structure...
This complementary base pairing is achieved by hydrogen bonding between the nucleobases. Owing to the high molecular recognition ability and programmability, DNA self-assembly has been extensively utilized to nanostructures and nanomaterials [1–3]. Over recent decades, a sustained effort has been ...
Figure 3. DNA has (a) a double helix structure and (b) phosphodiester bonds; the dotted lines between Thymine and Adenine and Guanine and Cytosine represent hydrogen bonds. The (c) major and minor grooves are binding sites for DNA binding proteins during processes such as transcription (the ...