The DNA strands have the opposite orientation: one strand is in the 5′ to 3′ direction with respect to the carbon atoms on the sugar (deoxyribose) and the complementary strand is in the 3′ to 5′ direction (Figure 1(a)). The two DNA strands are separated during DNA replication and...
DNA synthesis involves the hydrolysis of the inner phosphoric anhydride bond in a deoxynucleoside triphosphate (dNTP)—where N is usually either adenine (A), cytosine (C), guanine (G) or thymine (T)—and the formation of a new phosphodiester bond at the 3′-end of a DNA strand with the...
The synthesis of topologically non-trivial compounds requires the manipulation of molecular recognition with an extraordinarily high level of control. Now, DNA four-way junctions have been configured to construct synthetic DNA knots and links, which can then be used to investigate important DNA-processi...
All-in-One First-Strand Synthesis MasterMix (with dsDNase) 三代逆转录去基因组DNA一管化预混液 产品货号: EG15133S 产品规格: 100 rxns 产品价格: ￥1380.00 产品介绍 All-in-One First-Strand Synthesis MasterMix (with dsDNase) 是一款高效、便捷、减少污染的高质量一链 cDNA 合成试剂盒,包含热稳定的 ...
Hifair®AdvanceFast 1st Strand cDNA Synthesis Kit (No Dye)是基于Hifair®III 1st Strand cDNA Synthesis Kit (No Dye)开发的快速反转录试剂盒,适用于PCR扩增以及RT-qPCR实验。与Hifair®III 1st Strand cDNA Synthesis Kit (No Dye)相比,具有稳定的检出率、特异性、产量保证,且反转录总时长最快可低于6...
产品名称 Hifair® II 1st Strand cDNA Synthesis Kit 产品规格 100T 用途范围 仅做科研,不用于临床 是否进口 否 商品货号 11119ES 商品规格 25 mg 生产厂家 翌圣生物科技(上海)股份有限公司 规格编码 11119ES60 最小起订量 1.00 发货地 上海 售卖区域 全国 可售卖地 全国 类型 科研试剂 型号...
通过该复制,侵入链3'端不断延伸,供体双链中的同源链不断被置换,D环不断增长(此种修复性DNA复制被称为“链置换DNA合成反应”[strand displacement DNA synthesis reaction](98)),在DSBR初期被RecBCD降解掉的DNA序列重新得到合成。最终,延伸的侵入3'链与(由同一DSB造成的)另一DSE中的5'链连接,从而导致Holliday...
【15】The next step of transcription is calledpromoter clearance or promoter escape. RNA polymerase must clear the promoter once the first bond has been synthesized. The promoter is a DNA sequence that signals which DNA strand is tran...
凭借其独特结构,PolⅣ和PolV可对带有非编码损伤的DNA进行移损合成(translesion synthesis,TLS),具体方式为:“通过延伸在DNA损伤处形成的链滑移中间体跳过模板链上的DNA损伤”(PolⅣ倾向于此种方式)或“在模板链的DNA损伤处向合成链插入核苷酸(不依赖于碱基配对但仍具有模板依赖性),并对新形成的引物末端进行延伸(Po...
英文名 MightyScript Plus First Strand cDNA Synthesis Master Mix (gDNA digester) 相关类别 反转录PCR(RT-PCR) 储存 冷冻(-20℃) 编号 包装 库存 目录价(￥) 您的价格(￥) 数量 B639252-0100 100 PRE 现货 1140 1140产品描述 概述 MightyScript Plus第一链 cDNA 合成Master Mix(去基因组DNA)基于Ⅲ...