The nucleases, Exo1 and Dna2, degrade the single-strand DNA produced in the first step. RPA, which has high affinity for single-stranded DNA, binds the 3′ overhangs. The Rad51 protein then forms a filament of nucleic acid and protein on the single strand of DNA coated with RPA which ...
degradation of one strand of the double helix by enzymes known asnucleases. The latter method is often utilized duringDNA recombinationand repair; for example, inDNA mismatch repair, the strand containing an incorrectbaseis excised and then resynthesized with the complementary strand serving as ...
The DNA sequence that codes for a protein can be randomly mutated by modulating the PCR conditions such that DNA polymerase introduces mistakes into the newly polymerized DNA strand. Thereby, random changes in the amino acid composition of the encoded protein are generated. The technique is usually...
别 名 Chromosome 9 open reading frame 80; hSSBIP1; MISE; RP11-276E15.2; Sensor of single-strand DNA complex subunit C; Sensor of ssDNA subunit C; Single-stranded DNA-binding protein-interacting protein 1; SOSS C; SOSS complex subunit C; SOSS-C; SOSSC; SOSSC_HUMAN; SSB-interacting ...
DNA strand breaks. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Acts as a loading factor for POLB onto non-incised AP sites in DNA and stimulates the 5'-terminal deoxyribose 5'-phosphate (dRp) excision activity of POLB. Plays a...
Here, we report a single-cell method (scMspJI-seq) that enables strand-specific quantification of 5mC, allowing us to systematically probe the dynamics of global demethylation. When applied to mouse embryonic stem cells, we identified substantial cell-to-cell strand-specific 5mC heterogeneity, ...
15-5). Topoisomerase I relieves the strain by repeatedly breaking and rejoining one strand of the helix as it unwinds one turn, and thus is capable of relaxing either positive or negative supercoils. As helicase unwinds the helix, helix-destabilizing proteins bind to the single-stranded DNA...
46-1, blue loop], such as T·T in (CTG)n, G·G and A·A in (GGGA)n, or shared paired motifs [G·A in (GGA)n and the human TTAGGG telomeric sequence and GC·AA in other telomeric sequences], a specific arrangement characterized by interstrand, rather than intrastrand, stacking ...
2. The end-protection problem originates from the structural similarity between telomeres and DNA double-strand breaks, which can lead to recognition of the telomere by the DNA damage surveillance machinery2. When telomeres are falsely recognized as DNA damage, they are processed by the non-...
intrastrand crosslinks48(Fig.4c), a result that excludes the possibility that the CSA and CSB-dependent effects observed after FA exposure are due to these types of DNA–DNA crosslinks. Together, these data show that although all TC-NER factors are crucial for the repair of UV-induced and ...