Give a possible mRNA sequence that would for this peptide. What type of RNA copies the sequence of DNA bases in complimentary form? Which one is genetic material, DNA or RNA? What is non-genetic RNA? True or false? DNA has 5'-to-3' directionality but RNA does not. ...
The sequence is commonly denoted as AGACC. Transcription of DNA to form the corresponding mRNA proceeds from the 5′-end to the 3′-end of the RNA molecules. Normally, DNA resides in the cellular nucleus with two strands in the form of a helix. One strand of DNA would appear as shown...
However, DNA adducts can also be measured at sequence-specific loci of the genome, using Southern blotting based approaches. Many nucleobase modifications (e.g. UV-induced pyrimidine dimers, strand breaks, bulky adducts and ICL) can form lesions which, if not repaired, interfere with the ability...
More recently, we have attempted to confirm these results using a newly isolated gonadotrope-derived cell line, LβT2, which expresses the endogenous LHβ gene (27). This cell line expresses SF-1 mRNA on Northern analysis and produces SF-1, or an immunologically related protein, based on ...
The term "hybridization", as used herein, refers to any process by which a strand of nucleic acid binds with a complementary strand through base pairing. The term "hybridization complex", as used herein, refers to a complex formed between two nucleic acid sequences by virtue of the formatio...
The 3′ cassette (GFP-II) contains a partial (812 bp) internal GFP coding sequence and is therefore inactivated by 5′ and 3′ truncations. The two cassettes are separated by 3.7 kb. After transfection with a plasmid encoding the I-SceI, a unique double strand break induces homology-...