Here, after providing an outline of the programmable nuclease-assisted genome editing field, we review the increasingly diverse range of DNA, RNA, and protein components (e.g., nucleases and “nickases”) that, when brought together, underlie RGN-based genome editing in eukaryotic cells. ...
本研究作者鉴定出SLFN5的过表达抑制了HIV-1的复制并降低病毒mRNA水平,而内源性SLFN5缺失则促进HIV-1复制。ChIP-seq+ChIP-qPCR检测结果表明SLFN5通过与U5-R区域的两个序列结合,显著降低HIV-1长末端重复序列(LTR)的转录活性,从而抑制RNA PoL II向转录起始位点募集。 对转染SLFN5-Myc和NL4-3luc.RE-DNA的HEK293T...
从而调控生物个体的发育过程,疾病发生和环境应答等生命活动;circRNA是一类不具有5`cap和3`ployA,以共价键形成环形结构的ncRNA分子,部分circRNA含有miRNA应答元件,可充当竞争性内源RNA(competing endogenousRNA,ceRNA),在胞浆中以类似海绵一样吸附miRNA,进而解除miRNA对其靶基因的抑制作用,从而调节基因的表达。
DNA may also be sequenced for epigenetic studies—e.g., methylation analysis (also known as bisulfite sequencing or Bis-Seq) and DNA–protein interaction sequencing (commonly known as ChIP-Seq), which are not covered in this sec...
Mcm2(Mcm2-7解旋酶的一个亚基)通过Pola将亲组蛋白传递到复制DNA的滞后链(Molecular Cell, 2018)。但MCM2本身位于Leading链上,Polα只能合成RNA引物和大约30nt的DNA序列,表明仅凭目前已知的通路无法完成Lagging上的组蛋白回收,这一直...
一、RNA-Protein研究 1.RNA Pull Down Assay(1) 为了鉴定特定的RNA分子是否与某一种特定的蛋白结合,研究人员根据DNA-Protein实验中DNA Pull down的实验方法进行改进,使其在RNA上实现。 技术步骤如下: (1)设计末端携带T7 RNA Promoter的引物扩增DNA片段; ...
DNA/RNA/Protein提取试剂盒品牌 编号:WK126 规格:50次 品牌:百奥莱博 产品介绍: 本试剂盒适用于从同一细胞或组织样本中同时分离纯化基因组DNA、总RNA和总蛋白。本产品无需将样品分为3份来分别提取DNA,RNA和蛋白质,也无需先将纯化后的总核酸分为两份后再分别纯化DNA和RNA,因此可*限度的回收DNA,RNA和蛋白质,...
Genes encoding Argonaute and Dicer-like protein leading to RNA silencing in GUS gene (the gene that encodes β-glucuronidase) expression by inducing inactivation of small RNAs complementary sequences and cleavage of double-stranded RNA is one of these mechanisms (Shin et al., 2016). In this ...
DNA–protein crosslinks (DPCs) induced by aldehydes interfere with replication and transcription. Hereditary deficiencies in DPC repair and aldehyde clearance processes cause progeria, including Ruijs–Aalfs syndrome (RJALS) and AMeD syndrome (AMeDS) in
目标序列捕获法建库使用的探针设计有DNA探针和RNA探针,主要对目标区域进行靶向富集,确保样本目标序列完全富集,提高富集效率和覆盖的均一性,有效降低测序成本。基于扩增子的靶向NGS方法依赖多重PCR扩增步骤富集目标序列。实验过程中,基于扩增的...