Initiation, elongation and termination are three main steps inDNAreplication. Let us now look into more detail of each of them: Step 1: Initiation The point at which the replication begins is known as the Origin of Replication (oriC). Helicase brings about the procedure of strand separation...
Explain in simple terms the process of DNA replication. Describe DNA replication. Explain in general terms the process of DNA replication. Explain the process of DNA replication. Describe the steps of transcription and translation in a eukaryotic cell. ...
When the MCM motor of the replicative helicase becomes loaded onto chromatin, origin DNA is licensed for replication. Here, the authors show why the ATPase function of MCM is needed in this process. Powered by ATP hydrolysis, MCM steps away from its own loader, tracking along both DNA strands...
The double strands of DNA are separated, which requires the DNA helicase to disassemble the original double stranded DNA. Where the double chain breaks down is called a replication fork. Two replication forks can generate a replication bubble. The chemical mechanism of DNA replication is that the ...
Figure 1.Steps in concatemer formation and segregation from mother to bud cell.(a)Concatamers can be generated from mtDNA circles by two different mechanisms of replication and recombination: homologous DNA pairing-dependent rolling circle (RC) replication (in red) and RNA-priming dependent replicat...
Accurate chromosomal DNA replication is essential to maintain genomic stability. Genetic evidence suggests that certain repetitive sequences impair replication, yet the underlying mechanism is poorly defined. Replication could be directly inhibited by th
一、由单链断裂导致的复制叉坍塌(replication fork collapse)或由DNA复制受阻导致的复制叉断裂(replication fork breakage)是造成DSB的重要原因(复制叉坍塌或断裂几乎在E.coli的每个细胞周期中都会自然发生。在静止期E.coli中,复制叉可以R环为基础组建,并可在基因组中常见的自发单链断裂处坍塌。)。单链断裂可由单链...
The covalent joining of polynucleotides catalyzed by the DNA ligase is a necessary event in DNA repair, recombination, and most notably DNA replication which requires the joining of “Okazaki” fragments (the small, nascent ssDNA fragments generated from the copying of the minus strand). Initially...
DNA double-strand breaks (DSBs) can be caused by environmental agents such as radiation and drugs1,2,3and endogenous metabolism such as transcription and replication stress4,5. For example, normal metabolism has been estimated to cause ~1–50 DSBs per human cell per day6,7. Consequently, fas...
As the molecular properties of the replication fork are central to the regulation of replication5, we set out to investigate replication fork dynamics in totipotent cells in vivo and totipotent-like cells in culture. Results 2CLCs and totipotent embryos have a slow replication fork speed Totipotent...