DNA replication, also known assemi-conservative replication, is the process by which DNA is doubled. This is an important process taking place within the dividing cell. In this article, we shall discuss thestructureof DNA, the steps involved inDNA replication(initiation, elongation and termination...
When does DNA replication occur? Where does DNA replication occur? Learn about DNA polymerase and enzymes, DNA replication steps, and DNA...
The double strands of DNA are separated, which requires the DNA helicase to disassemble the original double stranded DNA. Where the double chain breaks down is called a replication fork. Two replication forks can generate a replication bubble. The chemical mechanism of DNA replication is that the ...
DNA replication(07-6)改 DNAReplication PARTIANOVERVIEW DNAReplication 1)Semi-conservativemechanism（半保留机制）2）Replicons(复制子),origins（复制起点）,andtermini（复制终点）3）Semi-discontinuousreplication（半不连续复制）4）RNApriming（引导）Thecharacteristicofreplication:Semi-conservativeSemi-discontinuous S...
Slowing down replication fork speed experimentally induces 2CLCs. In vivo, slowing fork speed improves the reprogramming efficiency of somatic cell nuclear transfer. Our data suggest that fork speed regulates cellular plasticity and that remodeling of replication features leads to changes in cell fate ...
replication peaks and Repli-seq revealed early replicated regions in K562 cells. The Wilcoxon Rank-sum test was applied for statistical analysis.dVenn diagram showing the overlapping peaks of E-B and EdU/HU in K562 cells. The cyan circle represents the total number of E-B peaks and the ...
b, DNA polymerases catalyse chain growth only in the 5፱ to 3፱ chemical direction, but both new daughter strands grow at the fork, so a dilemma of the 1960s was how the bottom strand in this diagram was synthesized. The asymmetric nature of the replication fork was recognized by the...
DNA double-strand breaks (DSBs) can be caused by environmental agents such as radiation and drugs1,2,3and endogenous metabolism such as transcription and replication stress4,5. For example, normal metabolism has been estimated to cause ~1–50 DSBs per human cell per day6,7. Consequently, fas...
DNA in cells is supercoiled and constrained into loops and this supercoiling and looping influence every aspect of DNA activity. We show here that negative supercoiling transmits mechanical stress along the DNA backbone to disrupt base pairing at specifi
It is not known, on the one hand, how chemical energy is transduced into mechanical motion, accounting for such large forces on sub-nanometer steps, and, on the other hand, how energy consumption in fidelity maintenance integrates in this non-equilibrium cycle. Here, we propose a translocation...