Because of DNA strand polarity, the bidirectional replication results in two distinct products, “leading” and “lagging” strands, according to the moving direction of the replication fork. The leading strand is synthesized as a single continuous chain, whereas the lagging strand is initia...
consists of two newly synthesized consists of two newly synthesized strands, and the parent duplex is strands, and the parent duplex is conserved. Dispersive replication conserved. Dispersive replication results in daughter duplexes that results in daughter duplexes that ...
In subject area: Neuroscience Eukaryotic DNA replication refers to the process by which eukaryotic cells duplicate their DNA, involving the binding of the origin recognition complex (ORC) to replication origins, the formation of pre-replicative complexes (pre-RCs), and the initiation of DNA replicati...
The separation of the two strands of parent DNA results in the formation of a replication fork. The active synthesis of DNA occurs in this region. The replication fork moves along the parent DNA as the daughter DNA molecules are synthesized. DNA helicases: These enzymes bind to both the DNA...
Despite extensive studies on DNA replication, the exchange mechanisms of DNA polymerase during replication remain unclear. Existing models propose that this exchange is facilitated by protein partners like helicase. Here we present data, employing a comb
【摘要】 DNA双链断裂(DSB)作为最为严重的一种DNA损伤类型,如不能及时、妥善的修复将极大的影响细胞基因组的稳定性,甚至导致细胞死亡。在 E.coli中,DSB可由外源性因素(如电离辐射、拟放射药物、拓扑异构酶…
High fidelity DNA ligation prevents single base insertions in the yeast genome DNA ligase 1 finalizes nuclear DNA replication by accurately sealing nicks generated during Okazaki Fragment Maturation. Here, Williams et al employ a low fidelity DNA ligase 1 variant to study mutation rates and specifici...
These strands are relevant as DNA replication begins in regions with a large number of AT base pairs. Interpretation of our results is aided by secondary-structure modelling where both the fractions of the different types of structures and the number of paired bases in the lowest-energy ones ...
It is thought that exposure of single-stranded DNA allows CAG·CTG repeats to form hairpins and slipped-strand DNA duplexes, as they do in vitro [1, 3, 4, 8, 9]. These secondary structures interfere with normal DNA metabolic processes or trigger aberrant ones, ultimately leading to ...
coli for DNA replication; it consists of at least 10 different subunits (including those of the core protein) and provides high processing ability for the enzyme. DNA polymerase III in DNA replication forms a dimeric holoenzyme complex consisting of two identical DNA polymerase III core enzymes to...