When the MCM motor of the replicative helicase becomes loaded onto chromatin, origin DNA is licensed for replication. Here, the authors show why the ATPase function of MCM is needed in this process. Powered by ATP hydrolysis, MCM steps away from its own loader, tracking along both DNA strands...
Finally, we sought to address the functional relevance of the replication dynamics and fork remodeling for reprogramming to totipotency. Terminally differentiated somatic cells can be reprogrammed to totipotency upon transplantation into enucleated oocytes51,52. However, this process is inefficient and ofte...
What are the steps that take place in DNA replication? Describe the steps involved in DNA replication for both the leading and lagging strands in a replication fork starting with the actions of helicase and topoisomerase. Explain in simple terms the process of DNA replication. ...
DNA molecule is the main carrier of biological genetic information, so its replication is of great significance in the life process. This review will focus on the process of DNA replication in detail, including its chemical structure, catalytic principle, various enzymes involved, spatial conformation...
[93]在体外用 14 种纯化的酶重构了 基于 oriC 的大肠杆菌基因组复制循环(图 1-a),并 证明了这种复制周期反应(replication-cycle reaction, RCR)可以选择性地扩增由多片段组装反应构建的 环状分子.OriCiro Genomics 基于此开发了一种无细 胞克隆系统,该系统通过多个重叠 DNA 片段的无缝 组装和 RCR 扩增,在一天...
“When WEE1 was inhibited in cells with a SETD2 mutation, the levels of deoxynucleotides, the components that make DNA, dropped below the critical level needed for replication,” noted Oxford’s Andy Ryan. “Starved of these building blocks, the cells die. Importantly, normal cells in the ...
cerevisiae centromeres are defined by an ~125-bp DNA sequence and termed "point centromeres" because of their simple organization (Figure 3A) [76]. Replication fork pausing was detected at centromeres of various chromosomes (CEN1, CEN3, CEN4 and CEN6), thus the ability to promote fork ...
Protein–DNA interactions play essential roles in several biological functions in cells, like gene transcription, DNA replication, repair and recombination. To perform their regulatory functions, many of the DNA-binding proteins, among which are transcription factors (TF), need to bind to specific dou...
The linear single-stranded piece is fully “unrolled” in the process. What happens next varies, depending on the circumstances. For simple plasmid replication, the unrolled old strand is used as a template to make a complementary strand. This double-stranded region is cut free and circularized...
Accurate chromosomal DNA replication is essential to maintain genomic stability. Genetic evidence suggests that certain repetitive sequences impair replication, yet the underlying mechanism is poorly defined. Replication could be directly inhibited by th