mRNA 纯化试剂盒/mRNA Isolation Kit 现货 钰博生物品牌 上海钰博生物科技有限公司 3年 查看详情 ￥20.00/盒 北京 组织RNA纯化试剂盒 脂肪组织总RNA提取 rna纯化/提取 优级品 诊断试剂 北京百奥创新科技有限公司 3年 查看详情 ￥30.00/盒 北京 外泌体纯化/提取试剂盒(血浆) Exosome extraction/purification 优...
methylome site frequency spectrum (mSFS) using worldwideArabidopsissamples, but they failed to find evidence for selection on genic CG epimutation under benign environments. The confounding effect between DNA variation and methylation variation, as well as the high-scaled epimutation rates become obstac...
Since the Phanta Max super-fidelity DNA polymerase (Vazyme Co.) can amplify large DNA up to 20 kb, we next used the plasmids with 17.3-kb in size as DNA templates and tested the efficacy of the SMLP method on site-directed mutagenesis. We constructed a pLV-U6-CMV-FLNA plasmid (about ...
试剂:qiaamp circulating nucleic acid kit(55114)、neb exonuclease v(m0345s)、minelute reaction cleanup kit(28206)、vazyme tn5(td501‑2)、nebnext 2xhifi pcr mix、agilent 2100及qubit4.0试剂。[0062]仪器:applied biosystems proflextm3x32‑孔pcr系统、roche‑lightcycler‑480、agilent 2100、qubit...
and the primers used for amplification as indicated: pSB-dGFP-F: TGAGTTTGGACAAACCACAAC; pSB-dGFP-R: GGTTGTGGCCATATTATCAT; For the generation of pSB-SFB-GFP vector, the SFB DNA sequences was inserted to pSB-V5-GFP vector using the ClonExpress II One Step Cloning Kit (Vazyme, China), ...
Molecular cloning was performed using either the ClonExpress Ultra One Step Cloning Kit (Vazyme, #C115-02) or CloneEZ PCR Cloning Kit (Genscript, #L00339). Amino-acid substitutions and deletion mutants were generated using PCR-directed mutagenesis. All construct sequences were verified by DNA ...
诺唯赞试剂说明书 高通量测序建库产品 TruePrep DNA Library Prep Kit.pdf,TD501-TD503 TruePrepTM DNA Library Prep Kit V2 for Illumina® Vazyme Biotech Co., Ltd Web: Tel: 400-600-9335 Vazyme biotech co., ltd. Sales: sales@ Support: support@ w 使用说明书 w S
Extraction of total RNA from transfected cell samples was performed using FastPure Cell/Tissue Total RNA Isolation Kit (Vazyme, China) according to the manufacturer’s instructions. The cDNA was generated using MonSciptTM RT III All-in-One Mix (with DNase) (Monad, China), according to the pro...
(R306R/V307V). To generate sgRNA-resistant CENP-N plasmid, the corresponding sgRNA PAM sequence in cDNA was eliminated through a synonymous mutation (V152V, GTG to GTA). All the mutations were generated by Mut Express II Fast Mutagenesis Kit (Vazyme) according to the manufacturer’s ...
(Vazyme, Cat.# N411) and fragmented into 200 ~ 500 bp lengths, then used for PCR amplification, adapter/index ligation, and DNA purification with a TruePrep® DNA Library Prep Kit V2 for Illumina (Vazyme, Cat.# TD502). BS-seq libraries were constructed using a previously reported...