Monarch®PCR & DNA Cleanup Kit Protocol 观看其他视频 产品信息 Monarch PCR & DNA 纯化试剂盒是一款快速可靠的纯化试剂盒,可从 PCR 和其它酶促反应中纯化多达 5 μg 高浓度和高纯度的 DNA。该产品中使用的结合/洗涤/洗脱流程,仅需极短的温育和离心时间。纯化柱可确保零缓冲液残留,零交叉污染,洗脱体积低至...
Refer to Page 1 for column design specifics in each kit Individual Kit Components Catalog No. Amount ADB (Agarose Dissolving Buffer) D4001-1-50 D4001-1-100 50 ml 100 ml DNA Wash Buffer (concentrate) DNA Elution Buffer Zymo-Spin™ I Columns (uncapped) D4003-2-6 D4003-2-24 D3004-...
“Spin” and “Vacuum” designations indicate the protocol used for genomic DNA isolation. The genomic DNA isolated with the Wizard® SV Genomic DNA Purification System is of high quality and performs well in agarose gel analysis, restriction enzyme digestions and PCR analysis as seen in Figure...
推荐使用 Thermo Scientific GeneRuler 50 bp DNA 分子量标准在琼脂糖或聚丙烯酰胺凝胶上对 50 bp 至 1,000 bp 范围内的双链 DNA 进行大小测定和近似定量。DNA 分子量标准由 13 DNA 片段组成,含有 6X TriTrack DNA 上样染料。 50 bp DNA 分子量标准的特点 ...
The GelRed or GelGreen nucleic acid dyes can be used with our DNA Ladders. Nucleic acid binding dyes can affect DNA migration during electrophoresis, therefore we highly recommend post-staining of gels. Please follow the gel staining protocol provided by the manufacturer of nucleic acid gel dyes...
Subsequently, the proteins were separated by size using SDS-PAGE gel electrophoresis. The gel was then transferred onto a PVDF membrane (0.22 μm, Invitrogen) through a process known as blotting for 3 h. Then, the membrane was cut due to different primary antibody incubation needs. The ...
Gel purify PCR products and assemble all fragments using a gene assembly protocol, based on Gibson assembly, such as NEBuilder® HiFiDNA AssemblyMaster Mix (NEBiolabs). After the final plasmid is validated by sequencing, proceed with transfer into amethanotrophstrain by conjugation orelectroporation...
The cell debris was then removed by centrifugation and the supernatant was run on a SDS-PAGE gel. Proteins were then transferred onto Immun-Blot® PVDF Membrane (Bio-Rad Laboratories, Inc.) and target proteins detected using rabbit Anti-Rhp51/Rad51 (1 in 2000 dilution) (Cosmo Bio Ltd, ...
The product is detected by PAGE (12–15%) in the presence of 8 M urea. 3. SUBSTRATE SPECIFICITIES i. Basic structure of DNA termini: nick sealing. DNA ligases join single-strand breaks with juxtaposed 3′-OH and 5′-P groups in a duplex DNA. Such single-strand breaks, called nicks,...
Looking for quick and dependable purification of your amplicon? Easilyclean up your PCR product, either from an agarose gel or directly from the reaction. Plan on using your amplicon for cloning? Seamlesslyinsert your PCR productinto any vector, at any site of linearization using ligation-independe...