Monarch®PCR & DNA Cleanup Kit Protocol 观看其他视频 产品信息 Monarch PCR & DNA 纯化试剂盒是一款快速可靠的纯化试剂盒,可从 PCR 和其它酶促反应中纯化多达 5 μg 高浓度和高纯度的 DNA。该产品中使用的结合/洗涤/洗脱流程,仅需极短的温育和离心时间。纯化柱可确保零缓冲液残留,零交叉污染,洗脱体积低至...
Sample amounts, sample throughput, protocol time, and size range of the libraries may determine the suitability of either method [20]. Size selection from agarose gels is essentially a gel purification process in which DNA fragment...
One to five micrograms of gDNA were sonicated to an approximate size range of 200–400 bp. Size selection was achieved by PAGE gel and yielded DNA fragments of 200–300 bp. DNA was quantified by fluorescent incorporation (Qubit, Invitrogen). The library preparation included end-repair, an...
DNA hybrids were incubated with the increasing amount of proteins in digestion buffer (15 mM Tris-HCl pH 7.5, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 10 mM MgCl2, 0.1 mg/mL BSA and 5% glycerol) for 5 min at room temperature, and then loaded onto 5% PAGE gels, the...
Procedure Overview Step 1: Bisulfite Conversion of DNA Step 2: Primer Design Step 3: MS-HRM Optimization Step 4: MS-HRM Standard Curve and Data Analysis Step 5: Sequencing the HRM Product Table References Abstract Downloa...
(dPAGE) (Southern, 2002). The gel electrophoresis glass plates are then disassembled and plastic wrap is placed on top to cover the gel to prevent contamination. Since the library has a covalent FAM label, the band can be visually observed under a blue light transilluminator. The bright band...
(1-2-3-4). When we run this out on a gel and then expose x-ray film to the gel, the only fragment we will see on the x-ray will be 1-2. The 3-4 fragment will be present in the gel, but because it does not contain the 5′ terminus of the original sequence, it does ...
Refer to Page 1 for column design specifics in each kit Individual Kit Components Catalog No. Amount ADB (Agarose Dissolving Buffer) D4001-1-50 D4001-1-100 50 ml 100 ml DNA Wash Buffer (concentrate) DNA Elution Buffer Zymo-Spin™ I Columns (uncapped) D4003-2-6 D4003-2-24 D3004-...
(site underlined above) which cuts the outside of its recognition site in the “N” region of the duplex. The digestion products were separated using a 12% native PAGE gel, and the radiolabeled P/T was recovered. The 3′-recessed terminus and 4 nt of 5′ single-stranded overhang were ...
The quantitative PCR (q-PCR) method is used for the quantitative analysis of the EWOD extracted cf-DNA. We used g-DNA (of known concentration; 100 ng) from the mouse tail to calculate our protocol’s extraction percentage. The g-DNA has undergone the extraction steps and we performed q-...