RNA在蛋白质合成过程中起着重要作用——其中转运核糖核酸，简称tRNA，起着携带和转移活化氨基酸的作用；信使核糖核酸，简称mRNA，是合成蛋白质的模板；核糖体的核糖核酸，简称rRNA，是细胞合成蛋白质的主要场所。此外，现在已知许多其他种类的功能RNA，如microRNA等。核酸类似物主要用于医学和分子生物学研究。历史 核酸的
Cost and time-efficient construction of a 3′-end mRNA library from unpurified bulk RNA in a single tube Article Open access 27 February 2024 References Levy, S. et al. The diploid genome sequence of an individual human. PLoS Biol. 5, 2113–2144 (2007). CAS Google Scholar Wheeler, ...
CONSTITUTION:A cDNA library of bacteriophage lambdagt11 prepared from human liver mRNA is screened with a monoclonal antibody (mixture of 15 kinds of antibodies) of anti-h-PCI (human-type protein c-inhibitor) prepared by a mouse. The selected clone is subjected to plaque hybridization using an...
2). Transcription factors alter the rate of transcription of DNA sequences into mRNA by binding to the DNA and increasing the ability of the transcriptional machinery responsible for formation of mRNA. This control mechanism for mRNA production may be the most important cause of variability in ...
Reverse transcriptase make cDNA copies of mRNA. These are valuable for making libraries from eukaryotic organisms since they do not contain any intron sequences. To make a cDNA library, the mRNA must be isolated and used as a template. The library generated therefore reflects only those genes ex...
FRETfluors—nanostructures with Cy3 and Cy5 dyes and a DNA scaffold—are used to generate distinct spectroscopic signals from different configurations and mixtures of mRNA, dsDNA and proteins in an anti-Brownian electrokinetic trap for single-molecule multiplexed sensing. ...
转录是基因表达为蛋白质的第一阶段。在转录中,mRNA(信使 RNA)中间体从 DNA 分子的一条链转录而来。 The RNA is calledmessenger RNAbecause it carries the "message," or genetic information, from the DNA to the ribosomes, where the infor...
hnRNP蛋白家族具有多种功能,他们在转录调节、前体mRNA剪接、mRNA输出、mRNA降解、核质穿梭、染色质重塑、端粒延长等多种生物过程中担当重要角色。hnRNPs在不用疾病中调节基因表达的作用越来越明确[3]。hnRNPs的表达水平在多种癌症中发生改变,也与各种神经退行性疾病相关。有研究表明hnRNP蛋白家族参与DNA损伤修复。
mRNA 被翻译的时候那样，同时有多个核糖体来翻译，在核内 DNA 上，这种拥堵相比起 mRNA 上的拥堵（...
“Messenger RNA (mRNA)” refers to the RNA that is without introns and that can be translated into protein by the cell. “cDNA” refers to a double-stranded DNA that is complementary to and derived from mRNA. “Sense” RNA refers to RNA transcript that includes the mRNA and so can be ...