(premixed) DNA-barrel structures were flown into the chamber and allowed to attach to the surface for 8 min. Finally, the imaging buffer with buffer B with dye-labeled imager strands was flowed into the chamber. Imager concentrations of the experiments can be found in Supplementary Tables6–...
this does not always causes functional disorders in the synthesized protein. For the mutation to affect the protein, the base replacement must produce some conformational alterations, loss of critical functional groups, or modify “key” sites in the molecule (such as active site of enzymes). Muta...
When copies of a DNA fragment over 1000 base pairs in length are labeled and hybridized in situ to denatured chromosomes on a microscope slide, they anneal to the complementary sequence in the target DNA, thus indicating the site of origin of the DNA fragment. The method is widely used in ...
The mechanical dysregulation of cells is associated with a number of disease states, that spans from fibrosis to tumorigenesis. Hence, it is highly desirable to develop strategies to deliver drugs based on the “mechanical phenotype” of a cell. To achie
PCR fragments of 100, 200, 300, 500 and 1,000 base pairs were purified using the Wizard® SV 96 PCR Clean-Up System on the Biomek® 2000 robotic workstation. Panel A. Agarose gel analysis. Purified (P) and unpurified (U) fragments were separated on an ethidium bromide-stained, 2% ...
目前已经获得成功应用的第三代测序技术主要有单分子实时测序技术(single molecule real time, SMRT)和纳米孔单分子测序技术(single-molecule nanopore DNA sequencing)。这类方法不需要PCR扩增,可以在单分子水平进行边合成边测序,通过直接检测原始状态的样本来获得核酸和核酸修饰的信息[105]。
英文名称:Southern DNA Marker(Labeled by Biotin) 保质期:长期 个月 保存条件:低温运输 品牌:百奥莱博 规格:20次 英文名:Southern DNA Marker(Labeled by Biotin) 产地:国产|进口 本产品是用生物素标记的lambda/Hind Ⅲ DNA Marker,可以用做Southern杂交的Marker,便于准确确定杂交片段的大小。
Probe: A labeled DNA sequence used to detect the presence of a complementary sequence in a sample. Target: The DNA sequence in a sample that is complementary to the probe. Denaturation: The process of separating the two strands of a double-stranded DNA molecule. Annealing: The process by whi...
Another approach to detect torsional stress of DNA, in the presence of altered Tw, relies on the properties of the molecule psoralen. Psoralen freely penetrates cellular membranes and intercalates preferentially between base pairs of negatively supercoiled DNA (Fig. 3.2A). Positively supercoiled DNA...
(v5) capture kit. Samples were run on the Illumina HiSeq 2000 sequencer that generates paired-end reads of up to 150 base pairs in length. Raw reads were mapped to thehuman genomev37 (GRCh37) reference genome using BWA-MEM (Li, 2013;Li and Durbin, 2009). Variants were called using...