DNA microarray chips: Fabrication and cutting-edge applicationsDNA microarrayNext generation sequencingSpatial transcriptomicsDigital data storageFor decades, DNA chips, also known as DNA microarrays, have been extensively utilized for high-throughput gene expression analysis and single nucleotide polymorphism (...
A flurry of manuals and technical monographs have appeared over the past few years to try and guide the novice investigator through the pitfalls of implementing microarray production in their laboratories and, more importantly, through the statistical minefield that accompanies the analysis of gene ...
& Lapp, H. Robust DNA microarray image analysis. Machine Vision and Applications 15, 11–28 (2003). https://doi.org/10.1007/s00138-002-0114-x Download citation Issue DateOctober 2003 DOIhttps://doi.org/10.1007/s00138-002-0114-x Share this article Anyone you share the following link with...
The labeled cDNAs from both the samples are placed in the DNA microarray so that each cDNA gets hybridized to its complementary strand; they are also thoroughly washed to remove unbounded sequences. Collection and analysis The collection of data is done by using a microarray scanner. This scanner...
The DNA microarray hybridization applications include the important areas of gene expression analysis and genotyping for point mutations, single nucleotide polymorphisms (SNPs), and short tandem repeats (STRs). In addition to the many molecular biological and genomic research uses, this review covers ...
DNA microarray, DNA chips, gene chips, or biochip is a collection of high-density microscopic single-stranded DNA attached to a solid surface by biochemical analysis. From:Modern Applications of Plant Biotechnology in Pharmaceutical Sciences,2015 ...
1.Microarray Expression Analysis:In this experimental setup, the cDNA derived from the mRNA of known genes is immobilized. The sample has genes from both the normal as well as the diseased tissues. Spots with more intensity are obtained for diseased tissue gene if the gene is over expressed in...
Although the chips detect RNAs that may or may not be translated into active proteins, scientists refer to these kinds of analysis as"expression analysis"orexpression profiling. Since there are hundreds or thousands of distinct probes on an array, each microarray experiment can accomplish the equival...
These smaller genomes, coupled with the importance of bacteria as causative agents of human, animal, and plant diseases, as well as their utility in industrial applications, have contributed to a large international effort yielding publicly available complete genome sequences for more than 40 ...
Learn what a microarray is and how DNA microarray analysis is performed. Understand how microarrays work and how gene expression is studied using...