Hence, three different kits, QIAamp blood mini kit, High Pure PCR Template Preparation Kit and the Puregene DNA isolation kit were evaluated on these aspects. Genomic DNA was isolated from whole blood samples with WBC counts ranging from 0.5 to 20*109 WBC/L. The WBC count was used to ...
Lahiri DK,Schuabel B.DNA isolation by a rapid method from human blood samples: Effects of MgCL2 , EDTA, storage time, and temperature on DNA yield and quality. Biochemical Genetics . 1993Lahiri DK, Sehuabel B. DNA isolation by a rapid method from human blood samples: Effects of MgCl2, ...
DNAexReagent用于从人和动物全血中快速抽提基因组DNA。全部操作过程不使用苯酚和氯仿,操作简单。所获得的基因组DNA长度在30-200 kb之间,可用于DNA存档,也可直接用于PCR相关实验、酶切等下游实验。 DNAex Reagent:4oC/室温保存。为悬浮液,用前混匀。 操作步骤和信息,都是人全血的。其它血液样品与此略有不同,请参...
Isolation of gDNA From Small Volumes of Whole Blood Using the Wizard Genomic DNA Kit In generally, the genome research of high DNA extraction from clotted blood produced a low quality. The aim of this study is to develop a simple and safe technique to dispering the blood clots by the ball...
(Figure 2.1). The exact extraction or DNA isolation procedure varies depending on the type of biological evidence being examined. For example, whole blood must be treated differently from a bloodstain or a bone fragment. James Stray and colleagues at Applied Biosystems/Life Technologies have ...
Cell-free DNA isolation from plasma Peripheral blood was collected from cancer patients using EDTA anticoagulant tubes. Plasma samples were isolated from whole blood using the UHN Biobank centrifugation protocol and stored at the UHN Biobank. 500 µl–1 ml plasma aliquots retrieved from the Bi...
blood 实验步骤 Protocol: DNA Purification from Blood or Body Fluids (Spin Protocol) This protocol is for purification of total (genomic, mitochondrial, and viral) DNA from whole blood, plasma, serum, buffy coat, lymphocytes, and body fluids using a ...
Establishment of a semi-automated pathogen DNA isolation from whole blood and comparison with commercially available kits Achieving reliable results using DNA based technologies is strongly dependent on pre-analytical processes including isolation of target cells and their DNA of ... H Wiesinger-Mayr,E...
The objective of this study was to develop an easy-to-perform method for the simultaneous isolation of RNA and DNA from the same sample. Due to the common nucleic acid structure RNA and DNA isolation procedures are very similar. The investigation of blood and semen stains has shown that DNA...
A DEP method has been developed which allows the rapid isolation, concentration and detection of DNA and nanoparticles directly from human and rat whole blood. Using a microarray device operating at 20 V peak-to-peak and 10 kHz, a wide range of high molecular weight (HMW)-DNA and ...