DNA复制的基本过程已经比较明晰：DNA复制起始于复制源，且每个复制源处同时形成两个姐妹复制叉（sister forks）【1】。姐妹复制叉反向行进，直至遇到对向行进的复制叉（convergent forks）触发DNA复制终止【2】。单细胞低等真核生物酵母是优秀的DNA复制研究模型，便于进行遗传筛选，因此DNA复制起始、延伸和终止的蛋白也被...
Moreover, problems encountered during DNA replication (replication stress) often result in under-replicated DNA that needs to be resolved during mitosis. Cells have developed a number of means to respond to these challenges, such as replication-coupled DNA repair pathways and S-phase checkpoint ...
Ligation efficiency is critical for conversion of DNA fragments into sequenceable molecules and thus impacts conversion rate and yield of the libraries. Because library fragments are flanked by adapters, they are sometimes called inserts...
2g,h). We conclude that 2CLC emergence occurs concomitant with DNA replication and that entry into the S-phase is key for this reprogramming. Fig. 2: Emergence of 2CLCs occurs together with or after DNA replication. a, Strategy to evaluate 2CLC emergence during the S-phase. b, After ...
This creates major problems in synchronization. Synthesis at each origin must be coordinated to make sure that each chromosome is completely replicated. Conversely, each origin must initiate once and once only during each replication cycle in order to avoid duplication of DNA segments that have ...
phase during the labeling, when nuclear DNA is replicated. Other cells show no incorporation of bromodeoxyuridine in their nuclear DNA and were in G1or G2of the cell cycle. All cells, however, show bromodeoxyuridine incorporation in granules in their cytoplasm, representing replicated mtDNA. Despite...
Cisco DNA Center 2.3.5.6 is generally available to all customers. Cisco DNA Center 2.3.5.6 contains important bug fixes, enhancements, and security updates. This document describes the features, limitations, and bugs for Cisco DNA Center, Release...
Polymerase chain reaction (PCR) is one of the most important developments in modern biotechnology. However, PCR is known to introduce biases, especially during multiplex reactions. Recent studies have implicated the DNA polymerase as the primary source of bias, particularly initiation of polymerization ...
In many eukaryotes, sequencing of cells at different stages of the cell cycle has been used to profile DNA replication timing, which measures the relative time that different genomic regions are replicated during S phase (reviewed in1). This replication-timing program is highly reproducible across ...
While the archival digital memory industry approaches its physical limits, the demand is significantly increasing, therefore alternatives emerge. Recent efforts have demonstrated DNA’s enormous potential as a digital storage medium with superior information durability, capacity, and energy consumption. Howeve...