Interstrand crosslinks (ICLs) represent a highly toxic form of DNA damage that can block essential biological processes including DNA replication and transcription. To combat their deleterious effects all eukaryotes have developed cell cycle-dependent repair strategies that co-opt various factors from '...
DNA链间交联（DNA interstrand crosslinks，ICLs）是DNA链共价连接的异常， 阻碍复制和转录的进行。FA修复途径包括22个FANC蛋白，比如BRCA1和BRCA2等。为了确定DNA链间交联修复缺陷背景下肿瘤形成的机制，并获得用于早期癌症检测的生物标记物，作者们对来自50位患者的55个独立的FA鳞状细胞癌以及3位腺癌患者的样本进行基因...
DNA链间交联（DNA interstrand crosslinks，ICL）是通过共价键连接两条DNA链，具有很高的细胞毒性，它能阻止DNA复制和转录。ICL形成剂通常用于肿瘤化疗，内源性细胞代谢产物也可以形成ICL。多项研究关注内源性修复ICL的机制，参与ICL内源性修复的蛋白包括结构特异性核酸内切酶，它们负责在ICL附近切开DNA；另一类属于跨损伤...
DNA链间交联(DNA interstrand crosslinks,ICL)是通过共价键连接两条DNA链,具有很高的细胞毒性,它能阻止DNA复制和转录。ICL形成剂通常用于肿瘤化疗,内源性细胞代谢产物也可以形成ICL。多项研究关注内源性修复ICL的机制,参与ICL内源性修复的蛋白包括...
DNA链间交联(DNA interstrand crosslinks,ICLs)是DNA链共价连接的异常, 阻碍复制和转录的进行。FA修复途径包括22个FANC蛋白,比如BRCA1和BRCA2等。为了确定DNA链间交联修复缺陷背景下肿瘤形成的机制,并获得用于早期癌症检测的生物标记物,作者们对来自50位患者的55个独...
Here, we report that adding interstrand crosslinks (ICLs)—substrates for the FA DNA repair pathway—to template DNA stimulates HDR by approximately threefold on a per mole basis in human cell lines, iPS cells and stimulated T cells, without increasing mutation frequencies or altering EJ repair ...
5. DNA interstrand crosslinks (ICLs) ICLs are highly cytotoxic lesions. In combination with UVA, 6-TG, S4T and the halothiopyrimidines all induce ICLs in duplex oligonucleotides in vitro [36], [62], [66], indicating that thionucleotides have the potential to form ICLs in vivo. ICLs in ...
1 ARTICLE NATURE COMMUNICATIONS | DOI: 10.1038/ncomms3338 DNA interstrand crosslinks (ICLs) are particularly toxic because they disrupt genetic information on both strands, potently inhibiting DNA replication and transcription. DNA ICLs are produced by many environmental agents including photoactivatable ...
他们的研究“PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks”发表在《The EMBO Journal》上,由Juan Méndez博士和CNIO的DNA复制小组领导。 研究人员发现,名为PrimPol的蛋白质是帮助这些肿瘤细胞逃避化疗的关键。 研究人员写道:“内源性醛类或化疗药物诱导的DNA链间交联(ICLs)...
研究人员发现,DDX11与9-1-1检查点复合蛋白合作对DNA修复起关键作用,正如其名,9-1-1的功能是检查DNA链复制后的完整性。FA通路可以改善链间交联(interstrand crosslinks,ICLs),ICLs是一种导致细胞死亡和发育障碍的严重有害损伤。 这一发现解释了暴露于ICLs下WABS和FA细胞之间的明显相似性,换句话说WABS亦可归类为FA...