See the kit user manual (G9700-90000) at www.agilent.com for more information. c For all samples with Cq DNA integrity score 1, use the Qubit-based gDNA concentration determined in step a, above, to determine volume of input DNA needed for the protocol. 48 SureSelect XT HS...
Initial quality control (QC) was performed to inspect the quality of imaging, binding density, and positive controls following the nSolver user manual. Data normalization was then performed using the NanoStringNorm package (v1.2.1) (Waggott et al., 2012). Specifically, endogenous probes were ...
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The DNA panel comprises 40 key genes while the RNA panel includes a broad fusion panel of 29 driver genes, enabling detection over 600 unique fusion isotypes. See flyer for gene targets (https://assets.thermofisher.com/TFS-Assets/CSD/Flyers/Oncomine_myeloid_assay_gx_flyer.pdf). Yes, we ...
WNT-surrogate-Fc fusion protein (Wnt-surrogate; ImmunoPrecise Antibodies; catalogue number N001, lot 5696, 6384, 7134, 7568) was dialysed against 25 mM HEPES and 500 mM NaCl buffer at pH = 8.2 using ZelluTrans/Roth Mini Dialyzer tubes MD300 (12–14 kDa, Carl Roth). Dialysis ...
Manual: Occurs when a super-admin user instructs Cisco DNA Center to swap the roles that are currently held by your system's active and standby sites. You would typically do this before you update the Cisco DNA...
Herculase II Fusion DNA PolymeraseCycling Conditions for Genomic or Vector DNA Targets ≤10 kbSegment Number of cycles TemperatureCatalog
Bound proteins were eluted with solution containing glutathione or imidazole and dialyzed against PBS buffer as the methods described in the user manual provided by the manufacturer. Immunofluorescence for DNA damage foci formation Cells were cultured on coverslips. After the desired DNA damage treatment...
The extracted DNA was amplified using PowerPlex® Fusion 6C System (Promega). 1.0 ng of template DNA was used for PCR amplification in a total reaction volume of 25 µl. 29 PCR cycles were used for amplifying the samples on Applied Biosystem® Veriti 96-Well Thermal Cycler (...
In a second PCR reaction, the 5' and 3' segments were fused, and the resulting fusion product was amplified in the same reaction. In this reaction, the given homologous regions (SspI-KpnI) annealed, effectively fusing the 5' and 3' segments, which at that time may have acted as their ...