Evaluation of DNA extraction methods for dried blood spots in the diagnosis of con- genital cytomegalovirus infection. J Clin Virol 2009; 46(Suppl 4):S37-42.de Vries JJ, Claas EC, Kroes AC, Vossen AC. Evaluation of DNA extraction methods for dried blood spots in the diagnosis of ...
Simple DNA Extraction Method for Dried Blood Spots and Comparison of Two PCR Assays for Diagnosis of Vertical Human Immunodeficiency Virus Type 1 Transmiss... Dried blood spots (DBS) on filter paper facilitate the collection, transport, and storage of blood samples for laboratory use. A rapid and...
Many studies reported optimisation of DNA extraction methods from environmental samples in the past three decades15,16,17,18,19; however, the type and diversity of environmental samples make it difficult to set a common method of DNA extraction. For instance, the basic principles for DNA ...
RapidExtractionofHighQualityDNAfromWholeBloodStoredat4ºCforLongPeriod MaterialsandMethods Standardchemicals Thismethodusesstandardchemicalsthatcanbeobtainedfromanymajorsupplier;weused chemicalssuppliedbySigmaCo.asfollow: EDTA(0.5M),pH8.0:Add186.1grofanhydrousEDTAto800mlofdistilledwater.AdjustpH to8.0withNaOHpel...
A simple, practical approach for the extraction of PCR-amplifiable DNA for the HLA-DQa gene from bloodstains deposited on various substrates is described. DNA from bloodstains is purified using Chelex 100 ion-exchange resin and then amplified. If amplification is not achieved, the extract is was...
1994). While the organic extraction method works well for recovery of high molecular weight DNA, it is time-consuming, involves the use of hazardous chemicals, and requires the sample to be transferred between multiple tubes, which increases the risk of error or contamination. When Millipore ...
三种血液基因组DNA提取方法的比较研究_李芳芳
英文名称:Blood DNA column extraction kit 产品规格:50次 发货周期:1~3天 本试剂盒是基于离心柱/硅胶膜原理的、专门用于从新鲜或冷冻的动物(包括人和禽类)抗凝全血中提取基因组DNA的试剂。 产品特点: 1. DNA纯净,OD260/280在1.8-2.0之间。不含蛋白质和RNA污染,可直接用于PCR、酶切、杂交等。
INTRODUCTION: Storing blood as dried spots on filter paper is a trustworthy approach used in genetic screening issues which justifies the necessity for a reliable DNA extraction method. The present work aims to investigate the effectiveness of superparamagnetic-bead based method in extracting DNA from ...
[关键词]DNA抽提;外周血;血细胞;DNA质量[中图分类号]R446.71[文献标志码]A[文章编号]0258—879X(2014)01—0101—05ThreemethodsforextractinghumangenomicDNAfromfreshandfrozenbloodsamples:acomparisonstudyJINJing,ZHULing,GAOJun,WUHong-yu,MANXiao-hua,ZHUYan-ping,GONGYan-fang,LIZhao-shen1.Departmentof...