Let’s set up a DNA extraction lab for kids. To get started, grab the free printable below. Pin STEP 1. Chill the alcohol in the freezer. STEP 2. Remove the green stems from the strawberries and add the strawberries to a zip lock bag. Make sure to seal the bag well and then squis...
Step 2: Your Individual DNA Extraction This is actually the easy part – where you send your swab or spit off to the lab and have it processed. All three of the main players utilize chip technology today. For example, 23andMe focuses on and therefore utilizes medical SNPs, where Family Tr...
The sequencing libraries were prepared using the same plasma extraction, library construction and hybrid capture-based methodology as FoundationACT with consistent analytical performance (that is, sensitivity, specificity) and has been previously described35,45. Briefly, between 1 ml and 5 ml of ...
Ancient DNA extractions Archival and archaeological samples underwent ancient DNA (aDNA) extraction in sterile, PCR-free conditions at the Ancient DNA Laboratory of the Department of Cultural Heritage (University of Bologna, Ravenna Campus, Italy), as part of another study which investigated their sp...
Two negative PCR controls (one from the aDNA lab and one from the PCR lab) and one positive control with synthetically reconstructed sequence were amplified in addition to the 41 DNA extracts from samples and six negative extraction controls, thus in total 50 samples/controls. The target region...
Extraction methods Commercial kits ●●●○○○□□□△ Lab protocols ○△ Download: Download high-res image (158KB) Download: Download full-size image Fig. 2. Recommended methods for sampling, preservation and extraction of sediment DNA depending on the target group of organisms. The choice of...
RNA extraction and cDNA synthesis were performed using the RNAprep Pure Kit (for tissue) and FastKing RT Kit (Tiangen Biotech). The hypoxanthine phosphoribosyltransferase (HPRT) gene was used as an internal control. The primers for HPRT and the five DMGs (BCKDHB, EPHX2, GOT2, RXRG, and ...
Drilling and extraction were done in designated labs physically separated from post-PCR laboratories, and where no previous work on red deer had been done before. Lab coats and breathing masks were used, gloves were changed frequently and drill bits were changed for each sample. Samples were ...
DNA extraction was performed using the NucleoSpin Tissue Kit (Macherey-Nagel) according to the manufacturer’s protocol. Quantitative PCR (qPCR) was performed using 50 ng or 1.5 µl of template DNA and 0.5 µM primers with the SYBR Green PCR Master Mix (Thermo Fisher Scientific)...
Egg extraction was performed 36 h later under the guidance of B-ultrasound. Fertilization was observed 16–18 h after insemination. Blastocysts were rated by the Gardner method, and D5 score ≥ 3BB or D6 score ≥ 4BB were defined as high-quality embryos. One to two high-quality ...