promega 组织和毛发提取试剂盒（与DNA IQ TM 系统结合使用）说明书 组织和毛发提取试剂盒（与DNA IQ TM系 统结合使用）操作手册 本说明书用于产品DC6740 所有的技术文献均可从公司网站www.promega.com得到 请访问公司网站以证实您所使用的技术手册为最新版本 I．介绍 (1)III. 产品组分 (2)III．组织和毛发提取...
One advantage this system has over other purification methods, such as phenol:chloroform extraction, is its ability to remove most inhibitors of amplification, including very small fragments of DNA. Tissue that has been stored in formalin for extended periods of time may be too cross-linked or ...
在 该方法基础上改进的针对微小组织和个体基因组DNA提取试剂盒还有德国Qiagen公司生产 的Qiagen DNeasy tissue extraction kit,美国Promega公司生产的genomic DNA purification kit等。商业试剂盒基本原理都是通过膜的吸附和洗脱完成对DNA的收集和 纯化,除了试剂价格昂贵以外,这种方法带来的问题主要是:1、由于小型浮游动物...
Masterpure™ Plant Leaf DNA purification kit This kit is specially designed for extraction and purification of DNA from of fresh plant-leaf tissue. Epicentre,USA Marsal et al. (2013) Invisorb Spin Plant Mini Kit This kit is designed for isolation of high quality total cellular DNA from diffe...
Wizard® Genomic DNA Extraction kit (WIZ) Detergent, RNase treatment, NaCl-containing buffer – – Isopropanol. Nucleospin (Clontech) Proteinase K, chaotropic salts – – Binding to a silica membrane – Wizard DNA Clean Up System (Promega) with proteinse K previous digestion Proteinase K, SDS...
美国Roche公司的DNAIsolationKitforCellandTissue和上海生工公司的 UNIQ-10小量柱式基因组DNA提取试剂盒对个体体积较小的圆紫菜(Porphyrasuborbiculato)、铁钉菜(Ishigeokamurae)等进行DNA的提取,获得高纯度的基因组DNA,完全能满足酶切片段长度多态性(AmplifiedFragmentLengthPolymorphism,AFLP)技术的需要。
STORAGE AND STABILITY: NukEx Mag RNA/DNA Kit components are shipped at ambient temperature. Kits must be stored at +18 to +25°C. Compare this product See the other productsLINEAR CHEMICALS solution reagent kitNukEx Mag Extreme SC for DNA extraction tissue solution reagent kit NukEx Mag...
使上述纯化dna在2%琼脂糖凝胶中电泳,切下约500～700bp的带后,使用gelextractionkit(qiagen社)从切下的凝胶提取、纯化dna。接下来,使用pgem-teasy载体系统(promega社)进行ta克隆,从而克隆上述dna。最后,通过碱制备法提取克隆的dna,通过桑格尔测序进行分析。分析使用bigdye(注册商标)terminatorv3.1cyclesequencingkit(...
Cell/Tissue Cells Maxwell® RSC Cultured Cells DNA Kit Maxwell® RSC Genomic DNA Kit Maxwell® RSC simplyRNA Cells Kit Tissue Maxwell® RSC Tissue DNA Kit Maxwell® RSC Genomic DNA Kit Maxwell® RSC simplyRNA Tissue Kit Maxwell® RSC miRNA Tissue Kit ...
DNA extraction was performed using the NucleoSpin Tissue Kit (Macherey-Nagel) according to the manufacturer’s protocol. Quantitative PCR (qPCR) was performed using 50 ng or 1.5 µl of template DNA and 0.5 µM primers with the SYBR Green PCR Master Mix (Thermo Fisher Scientific)...